Evaluación del potencial para inducir condrogénesis de soportes de colágeno II y cartílago hialino descelularizado, sembrados con condrocitos o células madre mesenquimales

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dc.contributor.advisorFontanilla Duque, Martha Raquelspa
dc.contributor.authorFlórez Cabrera, Adriana Matildespa
dc.contributor.researchgroupGrupo de Trabajo en Ingeniería de Tejidosspa
dc.date.accessioned2024-11-05T13:13:38Z
dc.date.available2024-11-05T13:13:38Z
dc.date.issued2022
dc.descriptionilustraciones, diagramas, fotografíasspa
dc.description.abstractEl cartílago hialino de la tráquea bovina puede ser empleado en la elaboración de soportes para aplicaciones en ingeniería de tejidos articulares. Este trabajo desarrolló, caracterizó y comparó soportes de colágeno II y cartílago hialino descelularizado, ambos hechos de tráquea bovina, pero con diferentes procedimientos. Para ello, se aisló colágeno II y se utilizó para elaborar soportes porosos, se estableció un método de descelularización de tráquea bovina y se obtuvieron soportes de cartílago hialino descelularizados. Se evaluaron y compararon las propiedades microestructurales, fisicoquímicas, mecánicas y biológicas de ambos tipos de soportes. También se evaluó y comparó el tejido formado cuando se sembraron condrocitos o células madre mesenquimales humanas (MSCs) en estos soportes, así como el perfil de factores angiogénicos secretados por los cultivos 3D resultantes. Las diferencias en composición, microestructura y propiedades mecánicas de los soportes afectaron la formación de tejido nuevo. En presencia de medio de diferenciación condrogénico, se observó cartílago hialino principalmente en los soportes de cartílago hialino descelularizado. Este hallazgo se correlaciona con la baja concentración de factores angiogénicos que se encuentran en el medio de cultivo de los soportes de cartílago hialino descelularizados sembrados con MSCs. Los resultados indican que los soportes de cartílago hialino descelularizados promueven el crecimiento y diferenciación condrogénica de condrocitos y MSC mejor que los soportes de colágeno II, los cuales favorecen la formación de tejido fibroso y fibrocartílago. Sin embargo, se necesita una evaluación preclínica de los soportes de cartílago hialino descelularizados en un modelo de lesión de cartílago para concluir sobre su biocompatibilidad (Texto tomado de la fuente).spa
dc.description.abstractHyaline cartilage from bovine trachea can be used to prepare scaffolds for joint tissue engineering applications. This work developed, characterized, and compared scaffolds of collagen II and decellularized hyaline cartilage, both made from bovine trachea but with different procedures. For this, collagen II was isolated and used to manufacture porous scaffolds, a decellularization method of bovine trachea was established and decellularized hyaline cartilage scaffolds were obtained. Microstructural, physicochemical, mechanical, and biological properties of both scaffolds were assessed and compared. It also assessed and compared the tissue formed when chondrocytes or mesenchymal stem cells (MSCs) were seeded on these scaffolds, as well as the profile of angiogenic factors secreted by the resulting 3D-cultures. Differences in composition, microstructure, and mechanical properties of scaffolds impacted the formation of new tissue. In the presence of chondrogenic differentiation medium, hyaline cartilage was observed mainly in decellularized hyaline cartilage scaffolds. This finding correlates with the low concentration of angiogenic factors found in the culture medium of decellularized hyaline cartilage scaffolds seeded with MSCs. The results indicate that decellularized hyaline cartilage scaffolds promotes chondrocyte and MSC growth and chondral differentiation better than collagen II scaffolds, which favor the formation of fibrous tissue and fibrocartilage. None of the less, a preclinical evaluation of the decellularized scaffolds in a cartilage injury model is still needed to conclude on their biocompatibility.eng
dc.description.degreelevelDoctoradospa
dc.description.degreenameDoctor en Ingenieríaspa
dc.description.methodsEn un trabajo previo, nuestro grupo de investigación estandarizó dos metodologías para el aislamiento de colágeno tipo II (doble digestión enzimática tripsina-pepsina y digestión enzimática solo con tripsina) a partir de tráquea bovina [20]. Los resultados del trabajo mencionado demostraron que las propiedades de los soportes son influenciadas por el método empleado para aislar el colágeno tipo II. Igualmente, que las características de los soportes producidos con el colágeno II aislado con tripsina lo pueden hacer útil como sustituto de cartílago articular al combinarse con técnicas de estimulación de la médula ósea.spa
dc.description.researchareaMateriales poliméricosspa
dc.format.extentxviii, 90 páginasspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.instnameUniversidad Nacional de Colombiaspa
dc.identifier.reponameRepositorio Institucional Universidad Nacional de Colombiaspa
dc.identifier.repourlhttps://repositorio.unal.edu.co/spa
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/87147
dc.language.isospaspa
dc.publisherUniversidad Nacional de Colombiaspa
dc.publisher.branchUniversidad Nacional de Colombia - Sede Bogotáspa
dc.publisher.facultyFacultad de Ingenieríaspa
dc.publisher.placeBogotá, Colombiaspa
dc.publisher.programBogotá - Ingeniería - Doctorado en Ingeniería - Ciencia y Tecnología de Materialesspa
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dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.licenseAtribución-NoComercial-SinDerivadas 4.0 Internacionalspa
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/spa
dc.subject.ddc620 - Ingeniería y operaciones afines::629 - Otras ramas de la ingenieríaspa
dc.subject.decsCartílago Hialinospa
dc.subject.decsHyaline Cartilageeng
dc.subject.decsColágeno Tipo IIspa
dc.subject.decsCollagen Type IIeng
dc.subject.decsCondrocitosspa
dc.subject.decsChondrocyteseng
dc.subject.decsCélulas Madre Mesenquimatosasspa
dc.subject.decsMesenchymal Stem Cellseng
dc.subject.proposalTráquea bovinaspa
dc.subject.proposalSoportes de colágeno IIspa
dc.subject.proposalSoportes de cartílago hialino descelularizadospa
dc.subject.proposalFactores angiogénicosspa
dc.subject.proposalBovine tracheaeng
dc.subject.proposalCollagen II scaffoldseng
dc.subject.proposalDecellularized hyaline cartilage scaffoldseng
dc.subject.proposalAngiogenic factorseng
dc.titleEvaluación del potencial para inducir condrogénesis de soportes de colágeno II y cartílago hialino descelularizado, sembrados con condrocitos o células madre mesenquimalesspa
dc.title.translatedEvaluation of the potential to induce chondrogenesis of collagen II and decellularized hyaline cartilage scaffolds, seeded with chondrocytes or mesenchymal stem cellseng
dc.typeTrabajo de grado - Doctoradospa
dc.type.coarhttp://purl.org/coar/resource_type/c_db06spa
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aaspa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/doctoralThesisspa
dc.type.redcolhttp://purl.org/redcol/resource_type/TDspa
dc.type.versioninfo:eu-repo/semantics/acceptedVersionspa
dcterms.audience.professionaldevelopmentEstudiantesspa
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2spa

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Doctorado en Ingeniería - Ciencia y Tecnología de Materiales