Impacto del tiempo de almacenamiento sobre el metabolismo de espermatozoides bovinos criopreservados

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dc.contributor.advisorRestrepo Betancur, Giovanni
dc.contributor.authorRueda Amador, Luis Andres
dc.contributor.cvlacRueda Amador, Luisspa
dc.contributor.googlescholarRueda Amador, Luisspa
dc.contributor.orcidRueda Amador, Luisspa
dc.contributor.researchgateRueda Amador, Luisspa
dc.contributor.researchgroupGrupo de investigación en biotecnología animal GIBAspa
dc.contributor.scopusRueda Amador, Luisspa
dc.date.accessioned2023-08-24T19:53:13Z
dc.date.available2023-08-24T19:53:13Z
dc.date.issued2023-02-15
dc.descriptionilustraciones, diagramasspa
dc.description.abstractSe ha observado que el almacenamiento del semen en condiciones de criopreservación afecta la viabilidad de los espermatozoides. Sin embargo, no está claro como el tiempo de almacenamiento afecta la calidad seminal. Algunas investigaciones han mostrado cambios en la cinética espermática a mayor tiempo de almacenamiento, mientras otros estudios no han mostrado efecto por periodos largos de almacenamiento. Sin embargo, las investigaciones diseñadas para identificar una disminución en el rendimiento del semen crioconservado en función del tiempo de almacenamiento son escasas. Por lo tanto, el objetivo de la investigación fue evaluar aspectos relacionados con el metabolismo y la integridad de espermatozoides bovinos criopreservados con diferentes tiempos de almacenamiento en nitrógeno líquido. Se evaluó la movilidad y cinética espermática con un sistema CASA-IVOS, la funcionalidad de la membrana mediante la prueba Host, la morfología con la tinción con eosina-nigrosina, la estabilidad de membrana con Merocianina 540, la actividad mitocondrial con DiOC6, el contenido de calcio intracelular con el indicador fluorescente Fura-2AM y la producción de especies reactivas de oxígeno (EROs) con DHR123 mediante citometría de flujo (FORTESSA LSR, BD Biosciences, EE. UU.). Para el análisis estadístico los datos se agruparon en tres periodos de almacenamiento (0-10, 11-20 y 21-30 años), se realizó el ajuste de modelos mixtos y se compararon las medias mediante la prueba de Tukey. Mediante un análisis de regresión se determinó la relación entre las variables dependientes y el tiempo de almacenamiento. No se encontraron diferencias en motilidad total y cinética espermática en relación con los grupos por tiempo de almacenamiento (p>0.05), mientras que, la motilidad progresiva fue mayor en el grupo de 11-20 años, en comparación con 0-10 años (p<0.05). Sin embargo, el análisis de regresión mostró la reducción de diferentes parámetros de movilidad y cinética, que fueron altamente dependientes del toro de proveniencia de las muestras seminales (p>0.05). Para HOST se observó que el grupo de 21-30 años tuvo la menor funcionalidad de la membrana (p<0.05). Se observó que la morfología de la cabeza fue diferente entre los grupos de 0-10 y 11-20 años (p<0.05), mientras que se observó mayor proporción de anormalidades totales y de la pieza media para el grupo de 21-30 años (p>0.05). Se encontró mayor proporción de espermatozoides M540-bajo en los grupos de 11-20 y 21-30 años de almacenamiento, siendo este último grupo, el que tuvo mayor proporción de células con alto flujo de calcio (Fura-Alto). La población de células con alto potencial de membrana mitocondrial (DiOC-Alto) fue mayor para el grupo de 11-20 años en relación con el grupo de 0-10 años (p>0.05). En conclusión, largos periodos de almacenamiento del semen bovino criopreservado pueden afectar negativamente la movilidad, cinética, integridad funcional de la membrana y morfología de los espermatozoides, sin embargo, lo anterior está condicionado por el efecto diferencial del individuo (toro). La estabilidad de la membrana plasmática, el flujo de calcio intracelular y la actividad mitocondrial de los espermatozoides sufren modificaciones atribuibles al efecto de largos periodos de almacenamiento en nitrógeno líquido. (Texto tomado de la fuente)spa
dc.description.abstractStorage of semen under cryopreservation conditions has been observed to affect sperm viability. However, it is not clear how storage time affects semen quality. Some research has shown changes in sperm kinetics with longer storage times, while other studies have shown no effect for long storage periods; however, research designed to identify a decrease in the performance of cryopreserved semen as a function of storage time are scarce. Therefore, the objective of the research was to evaluate aspects related to the metabolism and integrity of cryopreserved bovine spermatozoa with different storage times in liquid nitrogen. Sperm motility and kinetics were evaluated with a CASA-IVOS system, membrane functionality using the Host test, morphology with eosin-nigrosin staining, membrane stability with Merocyanine 540, content of intracellular calcium with the fluorescent indicator Fura-2AM, mitochondrial activity with DiOC6 and the production of reactive oxygen species (ROS) with DHR123, were assessed by flow cytometry. For the statistical analysis, the data were grouped into three storage periods (0-10, 11-20 and 21- 30 years), the adjustment of mixed models was carried out and the means were compared using the Tukey test. Using a regression analysis, the relationship between the dependent variables and storage time was determined. No differences were found in total motility and sperm kinetics in relation to the groups by storage time (p>0.05), while progressive motility was higher in the group of 11-20 years, compared to 0-10 years (p<0.05). However, the regression analysis showed the reduction of different motility and kinetic parameters, which were highly dependent on the bull from which the semen samples were obtained (p>0.05). For HOST, it was observed that the group of 21-30 years had the lowest membrane functionality (p<0.05). It was observed that head morphology was different between the groups of 0-10 and 11-20 years (p<0.05), and a higher proportion of total and midpiece abnormalities was observed for the 21-30 years group (p>0.05). A higher proportion of M540-low spermatozoa was found in the groups of 11-20 and 21-30 years of storage, being this last group, the one that had the highest proportion of cells with high calcium flow (Fura- High). The population of cells with high mitochondrial membrane potential (DiOC-High) was higher for the group of 11-20 years, compared to the group of 0-10 years (p>0.05). In conclusion, long periods of storage of cryopreserved bovine semen can negatively affect the motility, kinetics, functional integrity of the membrane and morphology of the sperm, however, this is conditioned by the differential effect of the individual (sire). The stability of the plasmatic membrane, the intracellular calcium flux and the mitochondrial activity of the sperm suffer modifications attributable to the effect of long periods of storage in liquid nitrogen.eng
dc.description.curricularareaÁrea Curricular en Producción Agraria Sosteniblespa
dc.description.degreelevelMaestríaspa
dc.description.degreenameMagister en Ciencias Agrariasspa
dc.description.methodsLa movilidad y la velocidad de los espermatozoides se evaluaron mediante el programa de análisis de semen asistido por computadora (CASA IVOS, Hamilton-Thorne, EEUU). Se colocó una gota de semen congelado-descongelado (7 μL) en un portaobjetos de vidrio precalentado (37°C), y se cubrió con un cubreobjetos. Se analizaron un mínimo de 500 espermatozoides (Microscopio, Eclipse E200, Nikon Inc., EE. UU.) Se determinaron la motilidad total (MT, %), la motilidad progresiva (MP, %), la velocidad media (VAP, μm/s), la velocidad de l nea recta (VSL, μm/s), la velocidad curvil nea (VCL, μm/s), el desplazamiento lateral de la cabeza (ALH, μm) y la frecuencia de batido (BCF, Hz). La funcionalidad de la membrana plasmática o integridad celular se evaluó mediante la técnica del Host (Jeyendran et al., 1984), el semen se incubó durante 30 minutos en solución de fructosa hipoosmótica a 100 mOsm/L en baño maría termoestable a 36°C. Para el análisis se realizó un frotis colocando 10 μL de la muestra, se observaron 100 espermatozoides utilizando un microscopio óptico. Los espermatozoides con cola enrollada total o parcialmente se consideraron con membrana integra (reaccionados) y el resultado final se expresó porcentualmente. Para la tinción con Eosina-Nigrosina se utilizó el método de Cabrera V. & Pantoja A., 2012, el semen se descongelo y se colocó en tubos eppendorf los cuales ya estaban incubados a 36°C, para el frotis se aspiraron 5 μL de Eosina y Nigrosina y 5μL de semen, se coloco sobre la lámina portaobjeto en la parte media de uno de los extremos y con ayuda de un tip se mezclaron los dos elementos, con ayuda de otra lamina con una ligera inclinación se distribuyó a lo ancho de la lámina para luego extender la muestra. Para el secado se colocó la lámina portaobjetos en una platina térmica a 36°C durante 15 min con el fin de secar rápidamente el frotis. Posteriormente el análisis se realizó contabilizando 100 células espermáticas. El conteo se hizo en distintos campos escogidos al azar con un microscopio óptico con aceite de inmersión a 100 X de aumento, la evaluación morfológica se realizó según clasificación de (Galloway, 1992), quien describe que los espermatozoides anormales se clasifican de acuerdo con la morfología, incluidos defectos en la cabeza como microcefalia, macrocefalia, cabeza piriforme, cabeza desprendida y/o rugosa, anomalías en la parte media como presencia de gota citoplasmática proximal o distal y colas dobladas o enrolladas. La estabilidad de la membrana plasmática o la alteración de la arquitectura de la membrana lipídica (asociada con la capacitación de los espermatozoides) se evaluó utilizando el protocolo adaptado de (Thomas et al., 2006), utilizando sondas fluorescentes Merocianina 540 (Molecular Probes Inc, Oregon, EE. UU). Se añadi 10 μL de semen diluido en medio DMSO a una concentración de 1 x 106 / ml. Por último, se agregó la sonda en concentraciones finales de 0.34 μL de Merocianina 540. Las muestras se incubaron durante 15 minutos a temperatura ambiente en la oscuridad. Luego, las muestras se evaluaron usando un citómetro de flujo (FORTESSA LSR, BD Biosciences, EE. UU.) en un rango de emisión entre 580-620 nm para sonda Merocianina 540. La merocianina 540 es un tinte hidrofóbico que puede monitorear la integridad de la membrana, especialmente la codificación de fosfolípidos cuya fluorescencia observada por citometria de flujo depende del grado de transtorno lipídico (Rodriguez-Martinez et al., 2001). Para evaluar el incremento del contenido de calcio intracelular, la concentración de calcio libre se evaluó utilizando el indicador de calcio fluorescente Fura-2AM siguiendo el protocolo de (Grynkiewicz et al., 1985). Las muestras se incubaron en 1 μL de Fura-2AM (F0888; Sigma-Aldrich) a 37°C durante 10 min. Luego, las muestras se evaluaron usando un citómetro de flujo (FORTESSA LSR, BD Biosciences, EE. UU.) en un rango de emisión entre 340-500 nm para la sonda Fura-2AM (Grynkiewicz et al, 1985 ; Brewis et al, 2000). Los datos fueron analizados con el software FlowJo versión 7.6.2 (FlowJo, LLC, USA). El fura-2 es un indicador de Ca2+ que contiene restos aromáticos, los cuales le confieren sus propiedades fluorescentes. Se trata de una molécula polar, y por ende, incapaz de atravesar las membranas celulares, por lo que es incluido en las células en forma de su derivado acetoximetiléster (fura-2/AM), el cual, gracias a su carácter hidrófobo atraviesa las membranas celulares mediante transporte pasivo (Grynkiewicz et al., 1985). Para la evaluaci n de la actividad mitocondrial, 10 μL de semen descongelado se incubaron en una soluci n con 300 μL de PBS, 0.5 μL de DiOC6 (0.1 μM) y 1 μL de yoduro de propidio por 15 min. El potencial de membrana mitocondrial (Δ¥M) fue medido a través de citometría de flujo y analizado con el software FlowJo versión 7.6.2 (FlowJo, LLC, USA). Para teñir las mitocondrias, cualquier sonda tiene que entrar en la célula y llegar a los orgánulos. Su acumulación citoplasmática es un evento crucial, ya que se requiere una concentración intracelular crítica para obtener una señal de fluorescencia adecuada, obviamente, para moléculas catiónicas lipófilas, dicha acumulación depende principalmente del potencial de membrana plasmática (Salvioli et al., 1997). La evaluación de la producción de EROs se realizó mediante la metodología del potencial de dihidrorodamina 123 (DHR123) (Kiani et al., 2012). Cada muestra se incubo con 1 μL de DHR123 a 37 °C durante 10 min. Luego, las muestras se evaluaron usando un citómetro de flujo (FORTESSA LSR, BD Biosciences, EE. UU.) en un rango de emisión entre 340- 500 nm para la sonda DHR123. Los datos fueron analizados con el software FlowJo versión 7.6.2 (FlowJo, LLC, USA). El tinte no fluorescente DHR123 es un derivado de la rodamina 123 (R123). Esta sonda entra pasivamente en las células y es oxidada por ROS para formar R123. R123 es un tinte fluorescente verde catiónico que puede acumularse y localizarse en las mitocondrias, Por lo tanto, DHR123 puede evaluar simultáneamente la presencia de producción de ROS en células con actividad mitocondrial (O’Connell et al., 2002).spa
dc.description.researchareaReproducción animalspa
dc.format.extentxvii, 89 páginasspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.instnameUniversidad Nacional de Colombiaspa
dc.identifier.reponameRepositorio Institucional Universidad Nacional de Colombiaspa
dc.identifier.repourlhttps://repositorio.unal.edu.co/spa
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/84599
dc.language.isospaspa
dc.publisherUniversidad Nacional de Colombiaspa
dc.publisher.branchUniversidad Nacional de Colombia - Sede Medellínspa
dc.publisher.facultyFacultad de Ciencias Agrariasspa
dc.publisher.placeMedellín, Colombiaspa
dc.publisher.programMedellín - Ciencias Agrarias - Maestría en Ciencias Agrariasspa
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dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.licenseReconocimiento 4.0 Internacionalspa
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/spa
dc.subject.ddc570 - Biología::573 - Sistemas fisiológicos específicos en animales, histología regional y fisiología en los animalesspa
dc.subject.ddc590 - Animales::599 - Mamíferosspa
dc.subject.ddc630 - Agricultura y tecnologías relacionadas::637 - Procesamiento lechero y productos relacionadosspa
dc.subject.ddc570 - Biología::572 - Bioquímicaspa
dc.subject.decsPreservación de semenspa
dc.subject.decsSemen Preservationeng
dc.subject.decsSemenspa
dc.subject.decsEspermatozoidesspa
dc.subject.decsSpermatozoaeng
dc.subject.decsPreservación de la fertilidadspa
dc.subject.decsFertility Preservationeng
dc.subject.lembReproducción dirigidaspa
dc.subject.lembReproduction controleng
dc.subject.proposalEvaluaciónspa
dc.subject.proposalEvaluationeng
dc.subject.proposalMetabolismospa
dc.subject.proposalMetabolismeng
dc.subject.proposalEspermatozoidesspa
dc.subject.proposalSpermeng
dc.subject.proposalCriopreservaciónspa
dc.subject.proposalCryopreservationeng
dc.subject.proposalTiempo de almacenamientospa
dc.subject.proposalStorage timeeng
dc.subject.proposalBovinospa
dc.subject.proposalBovineeng
dc.titleImpacto del tiempo de almacenamiento sobre el metabolismo de espermatozoides bovinos criopreservadosspa
dc.title.translatedImpact of storage time on the metabolism of cryopreserved bovine spermatozoaeng
dc.typeTrabajo de grado - Maestríaspa
dc.type.coarhttp://purl.org/coar/resource_type/c_bdccspa
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aaspa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/masterThesisspa
dc.type.redcolhttp://purl.org/redcol/resource_type/TMspa
dc.type.versioninfo:eu-repo/semantics/acceptedVersionspa
dcterms.audience.professionaldevelopmentInvestigadoresspa
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2spa
oaire.awardtitleImpacto del tiempo de almacenamiento sobre el metabolismo de espermatozoides bovinos criopreservadosspa
oaire.fundernameUniversidad Nacional de Colombiaspa

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