Evaluación de bacterias fermentadoras abe de suelo Rizosférico para producción de solventes

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dc.contributor.advisorGuerrero Fajardo, Carlos Alberto
dc.contributor.advisorCampos Rincon, Ivonne Maritza
dc.contributor.authorCastro Tibabisco , Karol Tatiana
dc.contributor.orcidCastro Tibabisco, Karol Tatiana [0000000344730606]
dc.contributor.researchgroupAprovechamiento Energético de Recursos Naturales
dc.date.accessioned2025-09-16T13:14:20Z
dc.date.available2025-09-16T13:14:20Z
dc.date.issued2025
dc.descriptionilustraciones a color, diagramas, fotografíasspa
dc.description.abstractEste estudio tiene como objetivo evaluar la producción de solventes (acetona, butanol y etanol) mediante fermentación ABE (Acetona-Butanol-Etanol) utilizando bacterias anaerobias aisladas de suelos rizosféricos de cultivo de papa Solanum tuberosum, a su vez se usaron residuos agroindustriales para evaluar la fermentación en los aislados y en 3 cepas de referencia conocidas. Se seleccionaron, se caracterizaron varias cepas, incluyendo Clostridium beijerinckii, Clostridium sacharoperacetonicum y Clostridium acetobutylicum ATCC 824, para su capacidad de producir solventes a partir de almidones extraídos de estos residuos agrícolas. La fermentación se realizó bajo condiciones anaerobias controladas, con una duración de 120 horas, cuantificándose los productos mediante cromatografía líquida (HPLC). Los resultados mostraron que el almidón de ñame fue el sustrato que generó mayores concentraciones de butanol, alcanzando hasta 4.5 g/L en las cepas más eficientes. Las cepas aisladas mostraron ser competitivas frente a las cepas de referencia, indicando el potencial de los residuos agrícolas como fuentes sostenibles para la producción de biocombustibles. Las conclusiones destacan la viabilidad de estos procesos en el contexto de la economía circular y la sostenibilidad industrial (Texto tomado de la fuente).eng
dc.description.abstractThis study aims to evaluate the production of solvents (acetone, butanol, and ethanol) through ABE (Acetone-Butanol-Ethanol) fermentation using anaerobic bacteria isolated from rhizospheric soils of potato crops (Solanum tuberosum). Additionally, agro-industrial residues were used to assess fermentation in the isolated strains as well as in three known reference strains. Several strains, including Clostridium beijerinckii, Clostridium saccharoperacetonicum, and Clostridium acetobutylicum, were selected and characterized for their ability to produce solvents from starches extracted from these agricultural residues. The fermentation was carried out under controlled anaerobic conditions for 120 hours, with the products being quantified using High-Performance Liquid Chromatography (HPLC). The results showed that yam starch was the substrate that produced the highest concentrations of butanol, reaching up to 4.5 g/L in the most efficient strains. The isolated strains demonstrated competitiveness compared to the reference strains, indicating the potential of agricultural residues as sustainable sources for biofuel production. The conclusions highlight the feasibility of these processes in the context of the circular economy and industrial sustainability.
dc.description.degreelevelMaestría
dc.description.degreenameMagister en Ciencias - Microbiología
dc.description.methodsEl área de estudio se desarrolló en la Unidad Agroambiental el Vergel en el municipio de Facatativá Cundinamarca con las coordenadas de 4°52'01.6"N 74°20'28.8"W perteneciente a la Universidad de Cundinamarca, esta finca ha sido usada para cultivo de papa como actividad académica y de investigación. El área usada para cultivo de papa fue la base del muestreo realizado.
dc.description.researchareaBiotecnología: Aprovechamiento de recursos
dc.format.extent144 páginas
dc.format.mimetypeapplication/pdf
dc.identifier.instnameUniversidad Nacional de Colombiaspa
dc.identifier.reponameRepositorio Institucional Universidad Nacional de Colombiaspa
dc.identifier.repourlhttps://repositorio.unal.edu.co/spa
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/88790
dc.language.isospa
dc.publisherUnivesridad Nacional de Colombia
dc.publisher.branchUniversidad Nacional de Colombia - Sede Bogotá
dc.publisher.facultyFacultad de Ciencias
dc.publisher.placeBogotá, Colombia
dc.publisher.programBogotá - Ciencias - Maestría en Ciencias - Microbiología
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dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.rights.licenseAtribución-NoComercial-SinDerivadas 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.ddc540 - Química y ciencias afines::547 - Química orgánica
dc.subject.lembINDUSTRIA DE ALMIDONESspa
dc.subject.lembStarch industryeng
dc.subject.lembINDUSTRIA DE PRODUCTOS VEGETALESspa
dc.subject.lembPlant products industryeng
dc.subject.lembDISOLVENTESspa
dc.subject.lembSolventseng
dc.subject.lembACETONAspa
dc.subject.lembAcetoneeng
dc.subject.lembALCOHOLspa
dc.subject.lembAlcoholeng
dc.subject.lembFERMENTACIONspa
dc.subject.lembFermentationeng
dc.subject.proposalFermentacion ABEspa
dc.subject.proposalBacterias anaerobiasspa
dc.subject.proposalAnaerobias facultativasspa
dc.subject.proposalFuente de Carbonospa
dc.subject.proposalAlmidonesspa
dc.subject.proposalAislamientospa
dc.subject.proposalPapaspa
dc.subject.proposalArracachaspa
dc.subject.proposalÑamespa
dc.subject.proposalABE fermentationeng
dc.subject.proposalAnaerobic bacteriaeng
dc.subject.proposalCarbon sourceeng
dc.subject.proposalStarcheseng
dc.subject.proposalIsolationeng
dc.subject.proposalPotatoeng
dc.subject.proposalYameng
dc.titleEvaluación de bacterias fermentadoras abe de suelo Rizosférico para producción de solventesspa
dc.title.translatedEvaluation of ABE-fermenting bacteria from rhizospheric soil for solvent productioneng
dc.typeTrabajo de grado - Maestría
dc.type.coarhttp://purl.org/coar/resource_type/c_bdcc
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.contentText
dc.type.driverinfo:eu-repo/semantics/masterThesis
dc.type.redcolhttp://purl.org/redcol/resource_type/TM
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dcterms.audience.professionaldevelopmentPúblico general
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2

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