Identificación molecular por medio del Gen COI de escarabajos de importancia sanitaria en la región avícola de Fusagasugá, Cundinamarca

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dc.contributor.advisorVargas Duarte, Jimmy Jolman
dc.contributor.advisorGómez Ramírez, Arlen Patricia
dc.contributor.authorGalindo Acosta, Camilo Andrés
dc.contributor.researchgroupControl Genetico en Salud Animalspa
dc.coverage.cityFusagasugá
dc.coverage.countryColombia
dc.date.accessioned2022-04-08T20:03:53Z
dc.date.available2022-04-08T20:03:53Z
dc.date.issued2021-04
dc.descriptionilustraciones, diagramas, fotografías, mapas, tablasspa
dc.description.abstractLas principales especies de escarabajos asociados a las instalaciones avícolas son Alphitobius diaperinus, Carcinops pumilio y Carcinops troglodytes y pueden actuar como reservorios y/o vectores de microorganismos. El objetivo de este trabajo fue identificar la población coleópteros presentes en granjas de pollo de engorde en una de las regiones avícolas más importantes del país como lo es Fusagasugá, Cundinamarca. Se tomaron muestras cada 7 días hasta el día 35, se realizó conteo de coleópteros y posteriormente se hizo una clasificación taxonómica y genómica con el uso del gen de ADN mitocondrial (mtDNA) citocromo oxidasa I (COI); también se realizó un estudio de poblaciones bacterianas presentes en A. diaperinus y en cama de los galpones con el uso del gen 16S rRNA. La clasificación taxonómica de los coleópteros dio como más frecuente a A. diaperinus con 95,1% y C. troglodytes con 4,9%. Del gen COI se obtuvo que A. diaperinus tiene una similitud del 98,68% con genes de referencia pertenecientes a la misma especie, C. troglodytes no posee un genoma de referencia con el cual hacer un comparativo, por lo tanto, el gen COI detectado en esta investigación se convierte en el primer reporte para la construcción del genoma de esta especie. El microbioma presente en A. diaperinus dio una alta presencia de bacterias de la familia Enterobacteriaceae, principalmente Salmonella enterica y Escherichia coli. En la cama de la unidad productiva muestreada, la familia con mayor presencia fue Staphylococcaceae. Los resultados de esta investigación corroboran la utilidad de las herramientas moleculares para la caracterización de insectos como complemento a las claves taxonómicas reportadas. Asimismo, los análisis del gen 16S rRNA demuestran la importancia que desempeñan estos coleópteros como reservorios de patógenos de interés en salud pública humana y veterinaria. Esta investigación también se destaca como el primer reporte de secuencias y microbiota de coleópteros asociados a granjas avícolas. (Texto tomado de la fuente)spa
dc.description.abstractThe main species of beetles associated with poultry facilities are Alphitobius diaperinus, Carcinops pumilio and Carcinops troglodytes and can act as reservoirs and / or vectors for microorganisms. The objective of this work was to identify the coleopteran population present in broiler chicken farms in one of the most important poultry regions of the country, such as Fusagasugá, Cundinamarca. Samples were taken every 7 days until day 35, a coleopteran content was made and later a taxonomic and genomic classification was made with the use of the mitochondrial DNA (mtDNA) cytochrome oxidase I (COI) gene; A study of bacterial populations present in A. diaperinus and in the litter of the houses was also carried out with the use of the 16S rRNA gene. The taxonomic classification of coleopterans gave the most frequent A. diaperinus with 95.1% and C. troglodytes with 4.9%. From the COI gene it was obtained that A. diaperinus has a similarity of 98.68% with reference genes belonging to the same species, C. troglodytes does not have a reference genome with which to make a comparison, therefore, the COI gene detected in this research becomes the first report for the construction of the genome of this species. The microbiome present in A. diaperinus gave a high presence of bacteria of the Enterobacteriaceae family, mainly Salmonella enterica and Escherichia coli. In the litter of the sampled productive unit, the family with the highest presence was Staphylococcaceae. The results of this research corroborate the usefulness of molecular tools for the characterization of insects as a complement to the reported taxonomic keys. Likewise, the analysis of the 16S rRNA gene shows the importance that these coleopterans play as reservoirs of pathogens of interest in human and veterinary public health. This research also stands out as the first report of sequences and microbiota of coleopterans associated with poultry farms.eng
dc.description.degreelevelMaestríaspa
dc.description.degreenameMagíster en Ciencias - Microbiologíaspa
dc.description.researchareaBiología molecular de agentes infecciososspa
dc.format.extentxx, 127 páginasspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.instnameUniversidad Nacional de Colombiaspa
dc.identifier.reponameRepositorio Institucional Universidad Nacional de Colombiaspa
dc.identifier.repourlhttps://repositorio.unal.edu.co/spa
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/81453
dc.language.isospaspa
dc.publisherUniversidad Nacional de Colomboiaspa
dc.publisher.branchUniversidad Nacional de Colombia - Sede Bogotáspa
dc.publisher.departmentInstituto de Biotecnología (IBUN)spa
dc.publisher.facultyFacultad de Cienciasspa
dc.publisher.placeBogota, Colombiaspa
dc.publisher.programBogotá - Ciencias - Maestría en Ciencias - Microbiologíaspa
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dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.licenseAtribución-SinDerivadas 4.0 Internacionalspa
dc.rights.urihttp://creativecommons.org/licenses/by-nd/4.0/spa
dc.subject.ddc570 - Biología::576 - Genética y evoluciónspa
dc.subject.proposalAvícolaspa
dc.subject.proposalAlphitobius diaperinus
dc.subject.proposalCarcinops troglodytes
dc.subject.proposalCitocromo Oxidasa I
dc.subject.proposal16sRNA
dc.subject.proposalEnterobacteriaceae
dc.subject.proposalSalmonella enterica
dc.subject.proposalEscherichia coli
dc.subject.proposalStaphylococcaceae
dc.subject.proposalPoultryeng
dc.subject.proposalAlphitobius diaperinuseng
dc.subject.proposalCarcinops troglodyteseng
dc.subject.proposalCytochrome Oxidase Ieng
dc.subject.proposal16sRNAeng
dc.subject.proposalEnterobacteriaceaeeng
dc.subject.proposalSalmonella entericaeng
dc.subject.proposalEscherichia colieng
dc.subject.proposalStaphylococcaceaeeng
dc.subject.unescoBiología molecularspa
dc.subject.unescoMolecular biologyeng
dc.subject.unescoVeterinariaspa
dc.subject.unescoAve de corralspa
dc.subject.unescoBiología celularspa
dc.titleIdentificación molecular por medio del Gen COI de escarabajos de importancia sanitaria en la región avícola de Fusagasugá, Cundinamarcaspa
dc.title.translatedMolecular identification through the COI gene of beetles of sanitary importance in the poultry region of Fusagasugá, Cundinamarcaeng
dc.typeTrabajo de grado - Maestríaspa
dc.type.coarhttp://purl.org/coar/resource_type/c_bdccspa
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aaspa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/masterThesisspa
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dc.type.versioninfo:eu-repo/semantics/acceptedVersionspa
dcterms.audience.professionaldevelopmentEstudiantesspa
dcterms.audience.professionaldevelopmentInvestigadoresspa
dcterms.audience.professionaldevelopmentMaestrosspa
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2spa

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