Caracterización genómica de factores de virulencia de aislados clínicos de Pseudomonas aeruginosa basados en WGS provenientes de un hospital de Bogotá, Colombia

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dc.contributor.advisorBarreto Hernández, Emiliano
dc.contributor.advisorLeal Castro, Aura Lucía
dc.contributor.authorOsorio Certuche, Nicole
dc.contributor.researchgroupEpidemiologia molecular Bioinformática Enfermedades infecciosasspa
dc.coverage.cityBogotá
dc.coverage.countryColombia
dc.date.accessioned2023-07-25T16:58:01Z
dc.date.available2023-07-25T16:58:01Z
dc.date.issued2023-01-30
dc.descriptionilustraciones, diagramasspa
dc.description.abstractEn humanos la infección por P. aeruginosa es controlada por múltiples factores de virulencia y es una de las causas más recurrentes y graves de infecciones asociadas a la atención en salud. Por lo tanto, el objetivo general de este estudio fue caracterizar los perfiles genómicos de virulencia en aislados clínicos de Pseudomonas aeruginosa provenientes de un hospital universitario de Bogotá- Colombia, utilizando la tecnología de secuenciación de genoma completo (WGS). Este estudio prospectivo se realizó durante los años 2019 y 2021, en donde se secuenciaron 54 aislamientos provenientes de 37 pacientes de los servicios de UCI, hospitalización y Unidad quirúrgica, utilizando las plataformas illumina y Oxford Nanopore. En los genomas se encontraron un total de 246 genes de virulencia, encontrando la presencia de genes de gran importancia en la virulencia de esta bacteria, como el gen pilA que se detectó en el 48,1% de los aislamientos y el gen algD en el 100%. Para las toxinas la prevalencia fue para exoU de (16,6%), exoT (92,5%), exoS (79,6%) y exoY (88,8%). Adicionalmente se encontró la presencia de 16 secuencio-tipos (ST) ya reportados y se encontraron 13 ST nuevos. En conclusión, el uso de tecnologías como WGS permitió determinar el perfil de virulencia de aislados clínicos de P. aeruginosa, logrando un acercamiento global a los perfiles de virulencia de los aislamientos clínicos de esta bacteria en el país, siendo el primer reporte de la prevalencia de más de 200 genes de virulencia en Colombia para P. aeruginosa. (Texto tomado de la fuente)spa
dc.description.abstractIn humans, P. aeruginosa infection is controlled by multiple virulence factors and is one of the most recurrent and serious causes of healthcare-associated infections. The aim of this study was to characterize the virulence genomic profiles in clinical isolates of Pseudomonas aeruginosa from a hospital in Bogotá-Colombia, using whole genome sequencing (WGS) technology. A prospective study was carried out during the years 2019 and 2021, where 54 isolates from 37 patients from both the ICU and hospitalization services, and operating rooms were sequenced, using the illumina and Oxford Nanopore platforms. A total of 246 virulence genes were found in the genomes, finding the presence of genes of great importance in the virulence of this bacterium, such as the pilA gene that was detected in 48.1% of the isolates and the algD gene that found in the 100% of them. For toxins, the prevalence was for exoU de (16.6%), exoT (92.5%), exoS (79.6%) and exoY (88.8%). Additionally, the presence of 16 sequence-types (ST) already reported and 13 new ST were found. In conclusion, the use of technologies such as WGS made it possible to determine the virulence profile of clinical isolates of P. aeruginosa, achieving a global approach to the virulence profiles of the clinical isolates of this bacterium in the country, being the first report of the prevalence of more than 200 virulence genes in Colombia for P. aeruginosa.eng
dc.description.degreelevelMaestríaspa
dc.description.degreenameMagíster en Ciencias - Microbiologíaspa
dc.description.researchareaBiología molecular de agentes infecciososspa
dc.format.extentXI. 79 páginasspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.instnameUniversidad Nacional de Colombiaspa
dc.identifier.reponameRepositorio Institucional Universidad Nacional de Colombiaspa
dc.identifier.repourlhttps://repositorio.unal.edu.co/spa
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/84264
dc.language.isospaspa
dc.publisherUniversidad Nacional de Colombiaspa
dc.publisher.branchUniversidad Nacional de Colombia - Sede Bogotáspa
dc.publisher.facultyFacultad de Cienciasspa
dc.publisher.placeBogotá,Colombiaspa
dc.publisher.programBogotá - Ciencias - Maestría en Ciencias - Microbiologíaspa
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dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.licenseAtribución-NoComercial 4.0 Internacionalspa
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/spa
dc.subject.decsInfección latentespa
dc.subject.decsLatent Infectioneng
dc.subject.proposalPseudomonas aeruginosaspa
dc.subject.proposalFactores de virulenciaspa
dc.subject.proposalInfecciones Asociadas a la Atención en Saludspa
dc.subject.proposalSecuencio-tiposspa
dc.subject.proposalVirulence factorseng
dc.subject.proposalHealthcare-associated infectionseng
dc.subject.proposalSequence-typeseng
dc.subject.proposalWGSeng
dc.titleCaracterización genómica de factores de virulencia de aislados clínicos de Pseudomonas aeruginosa basados en WGS provenientes de un hospital de Bogotá, Colombiaspa
dc.title.translatedGenomic characterization of virulence factors of clinical isolates of Pseudomonas aeruginosa based on WGS from a hospital in Bogotá, Colombiaeng
dc.typeTrabajo de grado - Maestríaspa
dc.type.coarhttp://purl.org/coar/resource_type/c_bdccspa
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aaspa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/masterThesisspa
dc.type.redcolhttp://purl.org/redcol/resource_type/TMspa
dc.type.versioninfo:eu-repo/semantics/acceptedVersionspa
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2spa

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