Determinación del impacto de IGF2 en la comunicación entre trofoblasto y las células dNK

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dc.contributor.advisorUmaña Pérez, Yadi Adrianaspa
dc.contributor.authorGuevara Prieto, Valentinaspa
dc.contributor.cvlacGuevara Prieto, Valentina [0000118585]spa
dc.contributor.orcidGuevara Prieto, Valentina 0000-0003-1535-615Xspa
dc.contributor.researchgroupGrupo de Investigación en Hormonasspa
dc.date.accessioned2024-10-31T19:43:57Z
dc.date.available2024-10-31T19:43:57Z
dc.date.issued2024-01-30
dc.descriptionilustraciones, diagramasspa
dc.description.abstractEn el proceso de implantación blastocística debe existir una comunicación continua entre las células de trofoblasto, las células dNK y otras poblaciones inmunes. Esta comunicación se centra en ejercer control inmunológico para mantener la receptividad materna. El aumento en la concentración de factores como el IGF2 se ha asociado a un incremento en la invasividad de las células trofoblásticas, siendo relevante estudiar los mecanismos que modulan la comunicación entre las células de trofoblasto y las células dNK en presencia de IGF2. Para esto se planteó un modelo in vitro, en el que se diferenciaron células pNK hacia un fenotipo tolerogénico similar a las células dNK (i-dNK) para co-cultivarlas con células HTR-8/SVneo. Se recogieron los medios condicionados de i-dNK (MidNK) para evaluar su efecto en la proliferación, invasión y migración de las células de trofoblasto estimuladas con IGF2 y los de células de HTR-8/SVneo estimuladas con IGF2 (MHIGF) para determinar los cambios en la expresión de marcadores de diferenciación en las células i-dNK. El análisis de los medios MHIGF muestra un incremento en la expresión de citoquinas relacionadas con la tolerogenia. Este efecto es suprimido en los medios de los co-cultivos, sugiriendo que la comunicación intercelular puede estar activando el papel regulador de las células dNK. Este resultado concuerda con los cambios en expresión génica y en cambios fenotípicos, que muestran la capacidad de las células i-dNK de ejercer regulación sobre las células de trofoblasto en respuesta al estímulo con IGF2. (Texto tomado de la fuente).spa
dc.description.abstractIn the process of blastocyst implantation there must be continuous communication between trophoblast cells, dNK cells and other immune populations at the maternal interphase. This communication focuses on exerting immunological control to maintain maternal receptivity. The increase in the concentration of factors such as IGF2 has been associated with an increase in the invasiveness of trophoblast cells. As a result, it is important to study the mechanisms that modulate the communication between trophoblast cells and dNK cells in the presence of IGF2. For this, an in vitro model was proposed, in which pNK cells were differentiated towards a tolerogenic phenotype similar to dNK cells (i-dNK) to co-culture them with HTR-8/SVneo cells. Conditioned media from i-dNK (MidNK) were collected to evaluate its effect on the proliferation, invasion and migration of trophoblast cells stimulated with IGF2 and those of HTR-8/SVneo cells stimulated with IGF2 (MHIGF2) to determine changes in the expression of differentiation markers in i-dNK cells. Analysis of MHIGF media shows an increase in the expression of cytokines related to tolerance. This effect is suppressed in the co-culture media, suggesting that intercellular communication may be activating the regulatory role of dNK cells. This result is consistent with the changes in gene expression and phenotypic changes, which show the ability of i-dNK cells to exert regulation on trophoblast cells in response to IGF2 stimulation.eng
dc.description.degreelevelMaestríaspa
dc.description.degreenameMagíster en Ciencias - Bioquímicaspa
dc.description.researchareaTrofoblasto como modelo predictivo de progresión tumoralspa
dc.format.extentxvi, 51 páginasspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.instnameUniversidad Nacional de Colombiaspa
dc.identifier.reponameRepositorio Institucional Universidad Nacional de Colombiaspa
dc.identifier.repourlhttps://repositorio.unal.edu.co/spa
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/87134
dc.language.isospaspa
dc.publisherUniversidad Nacional de Colombiaspa
dc.publisher.branchUniversidad Nacional de Colombia - Sede Bogotáspa
dc.publisher.facultyFacultad de Cienciasspa
dc.publisher.placeBogotá, Colombiaspa
dc.publisher.programBogotá - Ciencias - Maestría en Ciencias - Bioquímicaspa
dc.relation.indexedBiremespa
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dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.licenseAtribución-NoComercial-SinDerivadas 4.0 Internacionalspa
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/spa
dc.subject.ddc570 - Biología::572 - Bioquímicaspa
dc.subject.decsCélulas Asesinas Naturalesspa
dc.subject.decsKiller Cells, Naturaleng
dc.subject.decsReceptor IGF Tipo 2spa
dc.subject.decsReceptor, IGF Type 2eng
dc.subject.proposalNatural killerspa
dc.subject.proposalDeciduaspa
dc.subject.proposalEmbarazospa
dc.subject.proposalComunicación materno-fetalspa
dc.subject.proposalNatural killereng
dc.subject.proposalDeciduaeng
dc.subject.proposalPregnancyeng
dc.subject.proposalMaternal-fetal communicationeng
dc.subject.wikidatatrofoblastospa
dc.subject.wikidatatrophoblast celleng
dc.titleDeterminación del impacto de IGF2 en la comunicación entre trofoblasto y las células dNKspa
dc.title.translatedIGF2 impact in the communication between trophoblast and dNK cellseng
dc.typeTrabajo de grado - Maestríaspa
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Tesis de Maestría en Ciencias - Bioquímica
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Maestría en Ciencias - Bioquímica