Síntesis, caracterización y evaluación de la actividad antibacteriana de nanopartículas de cobre y plata contra el fitopatógeno Ralstonia solanacearum

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dc.contributor.advisorGonzález Almario, Adrianaspa
dc.contributor.advisorDuarte Ruiz, Álvarospa
dc.contributor.authorGarcía Suárez, Angélica Tatianaspa
dc.contributor.researchgroupNuevos Materiales Nano y Supramolecularesspa
dc.date.accessioned2024-01-30T16:44:43Z
dc.date.available2024-01-30T16:44:43Z
dc.date.issued2023-11-17
dc.descriptionilustraciones (algunas a color), diagramas, fotografíasspa
dc.description.abstractRalstonia solanacearum es una bacteria fitopatógena que afecta a un amplio rango de hospedantes de importancia agronómica entre los que se encuentra el plátano (Musa x paradisiaca), causando la enfermedad denominada Moko o Maduraviche que afecta un amplio rango de musáceas a nivel mundial, siendo este un producto hortícola de importancia en la alimentación humana y en la economía colombiana. Para el control de esta enfermedad se han utilizado diferentes pesticidas y antibióticos, pero la aplicación de estos productos no muestra resultados satisfactorios. En este estudio se sintetizaron dos tamaños diferentes de nanopartículas de plata [1-AgNPs] y [2-AgNPs] mediante reducción química empleando nitrato de plata, borohidruro de sodio y citrato de sodio, y para la síntesis de nanopartículas de cobre [1-CuNPs] y [2-CuNPs], cloruro de cobre y ácido L-ascórbico. Para la caracterización se emplearon técnicas como: Dispersión Dinámica de Luz (DLS), Espectroscopia Ultravioleta Visible (Uv-Vis), Espectroscopia Infrarroja (IR), Espectroscopia Raman, Difracción de Rayos X (DRX), Absorción Atómica de Llama (FAAS), Microscopia Electrónica de Barrido (SEM) y Microscopia Electrónica de Transmisión del Alta Resolución (HRTEM), con el fin de identificar la composición, distribución de tamaño y morfología. Posteriormente, a partir de un pseudotallo de plátano proveniente de un cultivo de Norte de Santander - Colombia, que presentaba síntomas de necrosis de haces vasculares y exudado bacteriano, se aisló e identificó R. solanacearum y se evaluó in vitro la actividad antibacteriana de las nanopartículas contra este fitopatógeno por el método de difusión en pozo y se determinó la concentración mínima inhibitoria (CMI). Como resultado se evidenció una mayor actividad antibacteriana para las nanopartículas de cobre demostrando su potencial para abordar los desafíos en el tratamiento de esta enfermedad. (Texto tomado de la fuente)spa
dc.description.abstractRalstonia solanacearum is a phytopathogenic bacterium that affects a wide range of hosts of agronomic importance, among which is the plantain (Musa x paradisiaca), causing the disease called Moko or Maduraviche that affects a wide range of musaceae worldwide, this being an important horticultural product in human nutrition and in the Colombian economy. For the control of this disease, different pesticides and antibiotics have been used, but the application of these products not show satisfactory results. Therefore, in this study, two different sizes of silver nanoparticles [1-AgNPs] and [2-AgNPs] were synthesized by chemical reduction using silver nitrate, sodium borohydride and sodium citrate, and for the synthesis of copper nanoparticles [1- CuNPs] and [2-CuNPs], copper chloride and L-ascorbic acid. For the characterization, techniques such as: Dynamic Light Scattering (DLS), Ultraviolet Visible Spectroscopy (Uv-Vis), Infrared Spectroscopy (IR), Raman Spectroscopy, X-ray Diffraction (XRD), Flame Atomic Absorption (FAAS), were used. Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM), in order to identify the composition, size distribution and morphology. Subsequently, from a plantain pseudostem from a crop in Norte de Santander - Colombia, which presented symptoms of necrosis of vascular bundles and bacterial exudate, R. solanacearum was isolated and identified and evaluated in vitro the antibacterial activity of the nanoparticles against this phytopathogen by the well diffusion method and the minimum inhibitory concentration (MIC) was determined. As a result, a greater antibacterial activity was evidenced for copper nanoparticles, demonstrating their potential to address the challenges in the treatment of this disease.eng
dc.description.degreelevelMaestríaspa
dc.description.degreenameMagíster en Ciencias- Químicaspa
dc.format.extentxviii, 133 páginasspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.instnameUniversidad Nacional de Colombiaspa
dc.identifier.reponameRepositorio Institucional Universidad Nacional de Colombiaspa
dc.identifier.repourlhttps://repositorio.unal.edu.co/spa
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/85527
dc.language.isospaspa
dc.publisherUniversidad Nacional de Colombiaspa
dc.publisher.branchUniversidad Nacional de Colombia - Sede Bogotáspa
dc.publisher.facultyFacultad de Cienciasspa
dc.publisher.placeBogotá, Colombiaspa
dc.publisher.programBogotá - Ciencias - Maestría en Ciencias - Químicaspa
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dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.licenseAtribución-NoComercial-CompartirIgual 4.0 Internacionalspa
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/spa
dc.subject.agrovocRalstonia solanacearumspa
dc.subject.agrovocBactericidasspa
dc.subject.agrovocBactericideseng
dc.subject.ddc540 - Química y ciencias afines::546 - Química inorgánicaspa
dc.subject.ddc630 - Agricultura y tecnologías relacionadas::632 - Lesiones, enfermedades, plagas vegetalesspa
dc.subject.lccMetal nanoparticleseng
dc.subject.lccNanopartículas metálicasspa
dc.subject.lembMarchitez bacteriana del plátanospa
dc.subject.lembBacterial blight (Plantain banana)eng
dc.subject.lembEnfermedades bacterianas de las plantasspa
dc.subject.lembBacterial diseases of plantseng
dc.subject.lembBacterias fitopatógenasspa
dc.subject.lembBacteria, Phytopathogeniceng
dc.subject.proposalNanopartículasspa
dc.subject.proposalAgente antibacterianospa
dc.subject.proposalMarchitez bacterianaspa
dc.subject.proposalRalstonia solanacearumspa
dc.subject.proposalFitopatógenospa
dc.subject.proposalNanoparticleseng
dc.subject.proposalAntibacterial agenteng
dc.subject.proposalBacterial wilteng
dc.subject.proposalPhytopathogeneng
dc.titleSíntesis, caracterización y evaluación de la actividad antibacteriana de nanopartículas de cobre y plata contra el fitopatógeno Ralstonia solanacearumspa
dc.title.translatedSynthesis, characterization and evaluation of the antibacterial activity of copper and silver nanoparticles against the phytopathogen Ralstonia solanacearumeng
dc.typeTrabajo de grado - Maestríaspa
dc.type.coarhttp://purl.org/coar/resource_type/c_bdccspa
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aaspa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/masterThesisspa
dc.type.redcolhttp://purl.org/redcol/resource_type/TMspa
dc.type.versioninfo:eu-repo/semantics/acceptedVersionspa
dcterms.audience.professionaldevelopmentBibliotecariosspa
dcterms.audience.professionaldevelopmentConsejerosspa
dcterms.audience.professionaldevelopmentEstudiantesspa
dcterms.audience.professionaldevelopmentInvestigadoresspa
dcterms.audience.professionaldevelopmentMaestrosspa
dcterms.audience.professionaldevelopmentMedios de comunicaciónspa
dcterms.audience.professionaldevelopmentPersonal de apoyo escolarspa
dcterms.audience.professionaldevelopmentPúblico generalspa
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2spa
oaire.awardtitleProyecto aprobado No. 45667, registrado en HERMESspa
oaire.fundernameUniversidad Nacional de Colombia - Resolución de la UGI Facultad de Ciencias - Sede Bogotáspa

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