Evaluación preclínica de la fracción butanólica de cálices de Physalis peruviana L. en modelo murino de artritis reumatoide

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dc.contributor.advisorOspina Giraldo, Luis Fernando
dc.contributor.authorUmaña Bautista, Stefany Rocio
dc.contributor.cvlacUmaña Bautista, Stefanyspa
dc.contributor.googlescholarUmaña Bautista, Stefanyspa
dc.contributor.orcidUmaña Bautista, Stefanyspa
dc.contributor.researchgateUmaña Bautista, Stefanyspa
dc.contributor.researchgroupPrincipios Bioactivos en Plantas Medicinalesspa
dc.contributor.scopusUmaña Bautista, Stefanyspa
dc.date.accessioned2023-07-31T20:41:56Z
dc.date.available2023-07-31T20:41:56Z
dc.date.issued2023
dc.descriptionilustraciones, diagramas, fotografíasspa
dc.description.abstractLa medicina tradicional se ha enfocado en estudiar los beneficios terapéuticos de plantas de la familia solanácea. En el caso de la planta Physalis peruviana L. se han descrito sus propiedades antiinflamatorias, antioxidantes e inmunomoduladoras. Sin embargo, en la actualidad no se ha reportado su uso en modelos murinos de inflamación crónica en artritis. Objetivo: Evaluar el posible efecto antiartrítico de la fracción butanólica de los cálices de Physalis peruviana L. en ratas Wistar hembras con artritis inducida por adyuvante completo de Freund (ACF) y colágeno. Método: La fracción butanólica de los cálices de Physalis peruviana L. se obtuvo a partir de la percolación con etanol de material seco, al cual se le hizo fraccionamiento con diferentes solventes hasta obtener la fracción butanólica. Se utilizó un modelo de inducción de artritis por adyuvante de Freund (ACF) y colágeno en ratas Wistar, evidenciando un pico de máxima inflamación el día 21. Los individuos fueron tratados desde el día 21 hasta el día 36 con dexametasona como medicamento patrón (1 mg/Kg/día) y la fracción butanólica (100, 50, 25 mg/Kg). La artritis fue evaluada a través de medición volumétrica de miembros posteriores (articulación del maléolo, zona plantar e interfalángica), evaluación radiográfica y análisis histopatológico. El efecto de la fracción butanólica sobre la inflamación se evaluó midiendo los niveles de interleucina 1 beta (IL-1β) y factor de necrosis tumoral alfa (TNFα); mientras que TBARs, la capacidad antioxidante (FRAP) y superóxido dismutasa (SOD) fueron medidos para evaluar el efecto de la fracción butanólica sobre el estrés oxidativo. Resultados: La fracción butanólica de cálices de Physalis peruviana L. no presentó diferencias significativas en la disminución de inflamación en comparación con el grupo patrón. Ninguno de los parámetros bioquímicos presentó diferencias significativas entre los grupos de investigación. La artritis inducida por ACF y colágeno no presentó involución de acuerdo con el análisis histopatológico y radiográfico. Conclusión: La fracción butanólica de cálices de Physalis peruviana L. (100, 50, 25 mg/Kg/día/VO) no presentó actividad inmunomoduladora ni antioxidante. Palabras clave: Physalis peruviana L., artritis, antioxidante, inmunomodulador, antiartrítico, erosión ósea y pannus. (Texto tomado de la fuente)spa
dc.description.abstractTraditional medicine has focused on studying the therapeutic benefits of plants from the Solanaceae family. In the case of the Physalis peruviana L plant, its anti-inflammatory, antioxidant and immunomodulation properties have been described. However, its use in models of chronic inflammation in induced arthritis in murine models has not been reported to date. Objective: To evaluate the possible antiarthritic effect of the butanolic fraction of the calyces of Physalis peruviana L. in female Wistar rats with arthritis induced by complete Freund's adjuvant (CFA) and collagen. Method: The butanolic fraction of the calyces of Physalis peruviana L. was obtained from the percolation with ethanol of dry material, which was fractionated with different solvents until obtaining the butanolic fraction. A Freund's adjuvant (FCA) and collagen induction model of arthritis was used in Wistar rats, evidencing a peak of maximum inflammation on day 21. Individuals were treated with dexamethasone as standard drug (1 mg/Kg/day) and the butanolic fraction. (100, 50, 25 mg/Kg) from day 21 to day 36. Arthritis was evaluated through volumetric measurement of hind limbs (malleolar joint, plantar and interphalangeal area), radiographic evaluation, and histopathological analysis. The effect of the butanolic fraction on inflammation was evaluated by measuring the levels of interleukin (IL-1β) and tumor necrosis factor (TNFα) while TBARs, antioxidant capacity (FRAP) and superoxide dismutase (SOD) were measured to assess the effect of the butanolic fraction on oxidative stress. Results: The butanolic fraction of calyces of Physalis peruviana L. does not present significant differences in the reduction of inflammation compared to the standard group. None of the biochemical parameters presented significant differences between the research groups. The arthritis induced by CFA and collagen does not present involution according to the histopathological and radiographic analysis. Conclusion: The butanolic fraction of calyces of Physalis peruviana L. (100, 50, 25 mg/Kg/day/VO) did not present immunomodulatory and antioxidant activity. Keywords: Physalis peruviana L., arthritis, antioxidant, immunomodulator, antiarthritic, bone erosion, pannus.eng
dc.description.degreelevelMaestríaspa
dc.description.degreenameMagíster en Ciencias - Farmacologíaspa
dc.description.methodsLa fracción butanólica de los cálices de Physalis peruviana L. se obtuvo a partir de la percolación con etanol de material seco, al cual se le hizo fraccionamiento con diferentes solventes hasta obtener la fracción butanólica. Se utilizó un modelo de inducción de artritis por adyuvante de Freund (ACF) y colágeno en ratas Wistar, evidenciando un pico de máxima inflamación el día 21. Los individuos fueron tratados desde el día 21 hasta el día 36 con dexametasona como medicamento patrón (1 mg/Kg/día) y la fracción butanólica (100, 50, 25 mg/Kg). La artritis fue evaluada a través de medición volumétrica de miembros posteriores (articulación del maléolo, zona plantar e interfalángica), evaluación radiográfica y análisis histopatológico. El efecto de la fracción butanólica sobre la inflamación se evaluó midiendo los niveles de interleucina 1 beta (IL-1β) y factor de necrosis tumoral alfa (TNFα); mientras que TBARs, la capacidad antioxidante (FRAP) y superóxido dismutasa (SOD) fueron medidos para evaluar el efecto de la fracción butanólica sobre el estrés oxidativo.spa
dc.description.researchareaPlantas con actividad antiinflamatoriaspa
dc.format.extent130 páginasspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.instnameUniversidad Nacional de Colombiaspa
dc.identifier.reponameRepositorio Institucional Universidad Nacional de Colombiaspa
dc.identifier.repourlhttps://repositorio.unal.edu.co/spa
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/84382
dc.language.isospaspa
dc.publisherUniversidad Nacional de Colombiaspa
dc.publisher.branchUniversidad Nacional de Colombia - Sede Bogotáspa
dc.publisher.facultyFacultad de Cienciasspa
dc.publisher.placeBogotá,Colombiaspa
dc.publisher.programBogotá - Ciencias - Maestría en Ciencias - Farmacologíaspa
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dc.relation.referencesVan Riel, P., Smolen, J., Emery, P., Kalden, J., Dougados, M., Strand, C., & Breedveld, F. (2004). Leflunomide: a manageable safety profile. Journal of Rheumatology Supplement, 71, 21–24.spa
dc.relation.referencesWorld Health Organization. (2004). The global burden of disease 2004 (Vol. 1). Retrieved from https://doi.org/10.1038/npp.2011.85.spa
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dc.relation.referencesYalamati, P., Bhongir, A., Karra, M., & Beedu, S. (2015). Comparative analysis of urinary total proteins by bicinchoninic acid and pyrogallol red molybdate methods. Journal of Clinical and Diagnostic Research: JCDR, 9(8), BC01-4. Retrieved from https://doi.org/10.7860/JCDR/2015/13543.6313.spa
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dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.licenseAtribución-NoComercial 4.0 Internacionalspa
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/spa
dc.subject.ddc610 - Medicina y salud::615 - Farmacología y terapéuticaspa
dc.subject.ddc610 - Medicina y salud::616 - Enfermedadesspa
dc.subject.lembBotánica médicaspa
dc.subject.lembMedicine, botaniceng
dc.subject.lembMateria médica, vegetablespa
dc.subject.lembMateria medica, Vegetablespa
dc.subject.lembPlantas medicinalesspa
dc.subject.lembMedicinal planteng
dc.subject.proposalPhysalis peruviana L.spa
dc.subject.proposalArtritisspa
dc.subject.proposalAntioxidantespa
dc.subject.proposalInmunomoduladorspa
dc.subject.proposalAntiartríticospa
dc.subject.proposalErosión óseaspa
dc.subject.proposalPannusspa
dc.subject.proposalModelo murino de artritisspa
dc.subject.proposalArthritiseng
dc.subject.proposalAntioxidanteng
dc.subject.proposalImmunomodulatoreng
dc.subject.proposalAntiarthriticeng
dc.subject.proposalBone erosioneng
dc.titleEvaluación preclínica de la fracción butanólica de cálices de Physalis peruviana L. en modelo murino de artritis reumatoidespa
dc.title.translatedPreclinical evaluation of the butanolic fraction of calyces of Physalis peruviana L. in a murine model of rheumatoid arthritiseng
dc.typeTrabajo de grado - Maestríaspa
dc.type.coarhttp://purl.org/coar/resource_type/c_bdccspa
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aaspa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/masterThesisspa
dc.type.redcolhttp://purl.org/redcol/resource_type/TMspa
dc.type.versioninfo:eu-repo/semantics/acceptedVersionspa
dcterms.audience.professionaldevelopmentBibliotecariosspa
dcterms.audience.professionaldevelopmentEstudiantesspa
dcterms.audience.professionaldevelopmentInvestigadoresspa
dcterms.audience.professionaldevelopmentMaestrosspa
dcterms.audience.professionaldevelopmentPadres y familiasspa
dcterms.audience.professionaldevelopmentPersonal de apoyo escolarspa
dcterms.audience.professionaldevelopmentPúblico generalspa
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2spa
oaire.awardtitleEvaluación preclínica de la fracción butanólica de cálices de Physalis peruviana L. en modelo murino de artritis reumatoidespa

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