Exploración de modificaciones estructurales de un antígeno de la proteína MSP1 de Plasmodium spp y su influencia en su propiedad de reconocimiento molecular por sueros murinos y humanos, de personas de áreas postconflicto en Colombia

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dc.contributor.advisorLozano Moreno, José Manuelspa
dc.contributor.authorMelo Velanida, Fredy Leonardospa
dc.contributor.researchgroupMimetismo Molecular de Los Agentes Infecciososspa
dc.coverage.countryColombiaspa
dc.date.accessioned2024-01-17T18:32:49Z
dc.date.available2024-01-17T18:32:49Z
dc.date.issued2023-11-07
dc.descriptionilustraciones a color, diagramas, fotografíasspa
dc.description.abstractEstudios realizados en el marco de investigación del grupo de mimetismo molecular de los agentes infecciosos, evaluaron la reactividad de sueros de áreas endémicas de Colombia (san juan de nepomuceno - bolívar, tierralta - córdoba, nuquí - chocó y tumaco - nariño) (SIVIGILA, instituto nacional de salud, 2018), frente a lisados de dos cepas de referencia, 3D7 y FCB2 de Plasmodium falciparum, donde se identificaron bandas de reconocimiento de la proteína MSP1 con movilidades relativas de 195, 83, 42 y 38 kDa, que permitieron identificar la reactividad de sueros frente a la proteína precursora MSP1 y sus fragmentos de procesamiento, lo que valida la importancia de este antígeno de superficie respecto a su papel inmunológico, perfilándola como una proteína blanco en la implementación de modificaciones tipo amida reducida, que promuevan y evidencien una protección efectiva de individuos expuestos a la infección natural. (Texto tomado de la fuente)spa
dc.description.abstractStudies carried out within the research framework of the molecular mimicry of infectious agents group, evaluated the reactivity of sera from endemic areas of Colombia (San Juan de Nepomuceno - Bolívar, Tierralta - Córdoba, Nuquí - Chocó and Tumaco - Nariño) (SIVIGILA, national institute of health, 2018), compared to lysates of two reference strains, 3D7 and FCB2 of Plasmodium falciparum, where recognition bands of the MSP1 protein were identified with relative mobilities of 195, 83, 42 and 38 kDa, which allowed the identification the reactivity of sera against the MSP1 precursor protein and its processing fragments, which validates the importance of this surface antigen with respect to its immunological role, profiling it as a target protein in the implementation of reduced amide type modifications, which promote and demonstrate effective protection of individuals exposed to natural infection.eng
dc.description.degreelevelMaestríaspa
dc.description.degreenameMagister en Ciencias - Farmacologíaspa
dc.description.researchareaCiencias Médicas y de la Salud - Biotecnología en Saludspa
dc.description.sponsorshipLa Convocatoria para el Apoyo a Proyectos de Investigación y Creación Artística de la Sede Bogotá de la Universidad Nacional de Colombia - 2019, tiene como objetivo fortalecer el desarrollo de la investigación y la creación artística, contribuir a la formación en procesos de investigación y extensión de estudiantes de pregrado y posgrado, y generar impacto mediante el reconocimiento y visibilidad de la Universidad Nacional de Colombia de la sede Bogotá, a través de la producción académica asociada al desarrollo de proyectos de investigación.spa
dc.format.extent[100] páginasspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.instnameUniversidad Nacional de Colombiaspa
dc.identifier.reponameRepositorio Institucional Universidad Nacional de Colombiaspa
dc.identifier.repourlhttps://repositorio.unal.edu.co/spa
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/85352
dc.language.isospaspa
dc.publisherUniversidad Nacional de Colombiaspa
dc.publisher.branchUniversidad Nacional de Colombia - Sede Bogotáspa
dc.publisher.facultyFacultad de Cienciasspa
dc.publisher.placeBogotá, Colombiaspa
dc.publisher.programBogotá - Ciencias - Maestría en Ciencias - Farmacologíaspa
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dc.relation.referencesEllis, R., Martin, L. B., Shaffer, D., Long, C., Miura, K., Fay, M., … Durbin, A. (2010). Phase 1 trial of the Plasmodium falciparum blood stage vaccine MSP1 42-C1/alhydrogel with and without CPG 7909 in malaria naïve adults. PLoS ONE, 5(1), 1–9. https://doi.org/10.1371/journal.pone.0008787spa
dc.relation.referencesEsen, M., Kremsner, P., Schleucher, R., Gässler, M., Imoukhuede, E., Imbault, N., … Mordmüller, B. (2009). Safety and immunogenicity of GMZ2 - a MSP3-GLURP fusion protein malaria vaccine candidate. Vaccine, 27(49), 6862–6868. https://doi.org/10.1016/j.vaccine.2009.09.011spa
dc.relation.referencesEspejo, F., Bermúdez, A., Vanegas, M., Rivera, Z., Torres, E., Salazar, L. M., & Patarroyo, M. E. (2005). Elongating modified conserved peptides eliminates their immunogenicity and protective efficacy against P. falciparum malaria. Journal of Structural Biology, 150(3), 245–258. https://doi.org/10.1016/j.jsb.2005.03.007spa
dc.relation.referencesEspejo, F., Cubillos, M., Mary Salazar, L., Guzmán, F., Urquiza, M., Ocampo, M., … Patarroyo, Mspa
dc.relation.referencesE. (2001). Structure, Immunogenicity, and Protectivity Relationship for the 1585 Malarial Peptide and Its Substitution Analogues (Vol. 113).spa
dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.licenseAtribución-NoComercial-CompartirIgual 4.0 Internacionalspa
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/spa
dc.subject.ddc610 - Medicina y salud::615 - Farmacología y terapéuticaspa
dc.subject.ddc610 - Medicina y salud::616 - Enfermedadesspa
dc.subject.decsSimulación del acoplamiento molecularspa
dc.subject.decsMolecular docking simulationeng
dc.subject.decsPlasmodium falciparumspa
dc.subject.decsPlasmodium bergheispa
dc.subject.decsAntígenos de protozoosspa
dc.subject.decsAntigens, protozoaneng
dc.subject.decsPlasmodium yoeliispa
dc.subject.decsMalaria-Tratamiento farmacológicospa
dc.subject.decsTécnicas Inmunológicasspa
dc.subject.decsImmunologic techniqueseng
dc.subject.proposalProteína MSP1spa
dc.subject.proposalPlasmodium falciparum 3D7spa
dc.subject.proposalPlasmodium berghei ANKAspa
dc.subject.proposalPlasmodium yoelii 17XLspa
dc.subject.proposalMalariaspa
dc.subject.proposalModificación amida reducidaspa
dc.subject.proposalAcoplamiento molecularspa
dc.subject.proposalMSP1 proteineng
dc.subject.proposalReduced amide modificationeng
dc.subject.proposalMolecular dockingeng
dc.titleExploración de modificaciones estructurales de un antígeno de la proteína MSP1 de Plasmodium spp y su influencia en su propiedad de reconocimiento molecular por sueros murinos y humanos, de personas de áreas postconflicto en Colombiaspa
dc.title.translatedExploration of structural modifications of an MSP1 protein antigen from Plasmodium spp and their influence on its molecular recognition property by murine and human sera from people from post-conflict areas in Colombiaeng
dc.typeTrabajo de grado - Maestríaspa
dc.type.coarhttp://purl.org/coar/resource_type/c_bdccspa
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aaspa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/masterThesisspa
dc.type.redcolhttp://purl.org/redcol/resource_type/TMspa
dc.type.versioninfo:eu-repo/semantics/acceptedVersionspa
dcterms.audience.professionaldevelopmentAdministradoresspa
dcterms.audience.professionaldevelopmentBibliotecariosspa
dcterms.audience.professionaldevelopmentConsejerosspa
dcterms.audience.professionaldevelopmentEstudiantesspa
dcterms.audience.professionaldevelopmentGrupos comunitariosspa
dcterms.audience.professionaldevelopmentInvestigadoresspa
dcterms.audience.professionaldevelopmentMaestrosspa
dcterms.audience.professionaldevelopmentMedios de comunicaciónspa
dcterms.audience.professionaldevelopmentPadres y familiasspa
dcterms.audience.professionaldevelopmentPersonal de apoyo escolarspa
dcterms.audience.professionaldevelopmentProveedores de ayuda financiera para estudiantesspa
dcterms.audience.professionaldevelopmentPúblico generalspa
dcterms.audience.professionaldevelopmentReceptores de fondos federales y solicitantesspa
dcterms.audience.professionaldevelopmentResponsables políticosspa
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2spa
oaire.awardtitleConvocatoria para el apoyo a proyectos de investigación y creación artística de la sede Bogotá de la Universidad Nacional de Colombia - 2019spa
oaire.fundernameUniversidad Nacional de Colombiaspa

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