Producción de un polímero tipo polihidroxialcanoato (PHA) empleando residuos de la producción de biodiesel
Cargando...
Archivos
Autores
Director
Tipo de contenido
Document language:
Español
Fecha
Título de la revista
ISSN de la revista
Título del volumen
Documentos PDF
Resumen
El aumento en la producción de biodiesel ha generado un exceso de glicerol y otros residuos disponibles para el desarrollo de la industria biotecnológica; estos constituyen materias primas útiles para la producción de polihidroxialcanoatos (PHAs). En este trabajo se evaluó el crecimiento de seis cepas acumuladoras de PHAs sobre glicerol USP y tres residuos provenientes de diferentes plantas de producción de biodiesel, lo cuál permitió concluir que Burkholderia cenocepacia 2G-57 es la que mejor utilizó dichas fuentes de carbono para su desarrollo. Se determinaron la concentración inicial de sustrato y el efecto de la limitación de nitrógeno sobre el crecimiento y la producción del polímero con esta cepa, para posteriormente realizar fermentaciones en lote y lote alimentado. La fermentación con alimentación continua utilizando el sustrato proveniente de BioD, rico en ácidos grasos, presentó los mejores resultados, alcanzando 4.51g/L de PHA con porcentaje de acumulación de 56.49% de biomasa a las 15 horas de fermentación, tiempo para el cual se alcanzó el máximo contenido de polímero.
Abstract. The increase in biodiesel production has generated an excess of glycerol and other residues available for development of the biotechnological industry, substrates useful for the production of polyhydroxyalkanoates (PHAs). Throughout this study, the growth of six PHAs accumulating strains on USP glycerol and three different residues originating from biodiesel production plants were evaluated, and Burkholderia cenocepacia 2G-57 was selected as that which best used these sources of carbon for its development. The initial substrate concentration and the effect of nitrogen limitation on the growth and production of the polymer with this strain has been determinating, to subsequently perform batch and fed-batch fermentations. The fermentation with continuous feed using wastes from BioD, the substrate rich in fatty acids showed the best results in this study, reaching 4.51 g/L of PHA with a biomass percentage accumulation of 56.49% at 15 hours of fermentation, hour in which the maxim accumulation of polymer was achieved.
Abstract. The increase in biodiesel production has generated an excess of glycerol and other residues available for development of the biotechnological industry, substrates useful for the production of polyhydroxyalkanoates (PHAs). Throughout this study, the growth of six PHAs accumulating strains on USP glycerol and three different residues originating from biodiesel production plants were evaluated, and Burkholderia cenocepacia 2G-57 was selected as that which best used these sources of carbon for its development. The initial substrate concentration and the effect of nitrogen limitation on the growth and production of the polymer with this strain has been determinating, to subsequently perform batch and fed-batch fermentations. The fermentation with continuous feed using wastes from BioD, the substrate rich in fatty acids showed the best results in this study, reaching 4.51 g/L of PHA with a biomass percentage accumulation of 56.49% at 15 hours of fermentation, hour in which the maxim accumulation of polymer was achieved.