Aislamiento de pseudomonas spp desulfurizadoras de dibenzotiofeno
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Silva, Edelberto
Pino, Julián
Acosta, Ana
Salazar, Tilse
Saboya, Marta
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2000
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Se implementó una metodología para el aislamiento de Pseudomonas spp. De muestras contaminadas con petróleo y sus derivados, el enriquecimiento en caldo BHI – Tween 80 permitió obtener el 43.155 de los aislados, el enriquecimiento en caldo BHI el 29.45% y la siembra directa el 27.40%. El 36.3% de los aislados degradan solamente DBT de los cuales el 27.4% proviene de aislamientos por enriquecimiento en BHI – Tween 80. Se seleccionaron ocho para evaluar el grado de desulfurización, encontrándose que se presentan mineralización a sulfatos del DBT entre el 0.5% y el 11%, pero al desarrollar el proceso mediante cometabolismo este porcentaje aumentó hasta el 25%.
An appropriate methodology was implemented to isolate Pseudomonas spp. Starting from polluted samples with petroleum and their derivates. The enrichment method in broth BHI – Tween 80 allowed to obtain the 43.15% of the isolated, the enrichment in broth BHI 29,45% and the direct sows 27.40%. The 36.3% of the isolated ones degrade only DBT of which 27.4%comes from enrichment in BHI –Tween 80 isolations. Eight isolates were selected to evaluate the desulfurization degree. The DBT desulfurization to sulfates was ranged between the 0.5 and 115, but when the process was developed by means of cometabolism this percentage was increased until 25%.
An appropriate methodology was implemented to isolate Pseudomonas spp. Starting from polluted samples with petroleum and their derivates. The enrichment method in broth BHI – Tween 80 allowed to obtain the 43.15% of the isolated, the enrichment in broth BHI 29,45% and the direct sows 27.40%. The 36.3% of the isolated ones degrade only DBT of which 27.4%comes from enrichment in BHI –Tween 80 isolations. Eight isolates were selected to evaluate the desulfurization degree. The DBT desulfurization to sulfates was ranged between the 0.5 and 115, but when the process was developed by means of cometabolism this percentage was increased until 25%.