Determinación de niveles de corticosterona plasmática y pilosa en ratas wistar adolescentes hacinadas
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González Uarquin, Duván Fernando
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En el presente trabajo se pretende determinar si hay cambios en la concentración de corticosterona en plasma y pelo de ratas Wistar en etapa juvenil sometidas a condiciones de hacinamiento como modelo de estrés crónico. Se utilizaron 18 ratas Wistar machos de 38 días de edad, distribuidas en dos grupos, control y experimental. Los sujetos experimentales se sometieron a un periodo de hacinamiento durante la fase final de la etapa juvenil (27 días). Los sujetos hacinados presentaron disminución significativa P0,05 en el peso final promedio (266,5 ± 9,63g) respecto a los controles (341,2 ± 23,52g), además presentaron aumento significativo P0,05 en los niveles promedio de corticosterona plasmática (470,16 ± 113,24 ng/ml) y pilosa (40,5 ± 9,75pg/mg) frente a los niveles hallados en los controles (232,6 ± 78,72 ng/ml y 19,67 ± 2,57 pg/mg en plasma y pelo respectivamente). Este estudio contribuye al desarrollo científico en la búsqueda de métodos no invasivos para el monitoreo de la corticosterona.
Abstract. For this study we determine whether exist changes in levels of plasma and hair corticosterone of young Wistar rats under crowded conditions like chronic stress model. 18 Wistar rats of 38 days old were divided in two groups, control and overcrowding; the experimental subjects were exposed to an overcrowding period during their youth (Post natal day 65). The overcrowded subjects showed significant difference P0, 05 for the final body weight average (266,5 ± 9.63g) compared to control animals (23.52 ± 341,2g). Additionally, the overcrowded group showed higher corticosterone plasma concentration (470, 16 ± 113.24 ng / ml) and also hair corticosterone concentration (40, 5 ± 9.75 pg / mg) compared to control subjects (232, 6 ± 78.72 ng / ml and 19.67 ± 2.57 pg/ml in plasma and hair respectively). This work contributes to the search of noninvasive methods and the monitoring of corticosterone levels.
Abstract. For this study we determine whether exist changes in levels of plasma and hair corticosterone of young Wistar rats under crowded conditions like chronic stress model. 18 Wistar rats of 38 days old were divided in two groups, control and overcrowding; the experimental subjects were exposed to an overcrowding period during their youth (Post natal day 65). The overcrowded subjects showed significant difference P0, 05 for the final body weight average (266,5 ± 9.63g) compared to control animals (23.52 ± 341,2g). Additionally, the overcrowded group showed higher corticosterone plasma concentration (470, 16 ± 113.24 ng / ml) and also hair corticosterone concentration (40, 5 ± 9.75 pg / mg) compared to control subjects (232, 6 ± 78.72 ng / ml and 19.67 ± 2.57 pg/ml in plasma and hair respectively). This work contributes to the search of noninvasive methods and the monitoring of corticosterone levels.