Caracterización molecular de aislamientos de klebsiella pneumoniae portadores del genbla NDM-1 procedentes de una unidad neonatal en un hospital de Bogotá
Autores
Moncada Guayazán, María Victoria
Director
Valenzuela de Silva, Emilia María (Thesis advisor)
Tipo de contenido
Trabajo de grado - Maestría
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EspañolFecha de publicación
2014
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El estudio de 6 aislamientos de Klebsiella pneumoniae resistentes a carbapenémicos aislados en pacientes de una unidad neonatal y enmarcado en un sistema de vigilancia epidemiológica a nivel hospitalario, logró identificar por primera vez en nuestro país y Suramérica aislamientos productores de la metalo-β-lactamasa tipo NDM-1. El propósito de este estudio fue determinar las características moleculares y genéticas en dichos aislamientos; así como, caracterizar las posibles estructuras de movilización responsables de la emergencia y/o diseminación de este gen en nuestro país. Análisis moleculares mostraron que todos los aislamientos presentan el gen blaNDM-1 y genes en casete aadB-catB3 en un plásmido conjugativo ≥170 Kb del grupo de incompatibilidad A/C. Fueron relacionados clonalmente mediante PFGE y la tipificación de secuencias de múltiples locus mostró que todos los aislamientos son ST1043, diferente a los ST reportados previamente en NDM, sugiriendo la emergencia de NDM en nuestro país de manera distinta a lo encontrado con Klebsiella pneumoniae productora de KPC, el cual se debe casi exclusivamente a la diseminación del clon ST258
Abstract: The study of six isolates of carbapenem-resistant Klebsiella pneumoniae in patients of a neonatal unit and framed in a surveillance system in hospitals, identified for the first time in our country and South America isolates producing the metallo-β-lactamase type NDM- 1. The purpose of this study was to determinate the molecular and genetic features in these isolates; as well as characterize potential mobilizing structures responsible for the emergence and / or spread of this gene in our country. Molecular analysis showed that all the isolates have the blaNDM-1 gene and gene cassette array aadb-catB3 in a conjugative plasmid ≥ 170 kb of the incompatibility group A / C. Were clonally related by PFGE and multilocus sequence typing showed that all isolates were ST1043, unlike the ST previously reported in NDM, suggesting the emergence of NDM in our country differently from what have found in KPC producing Klebsiella pneumoniae, which is almost entirely due to the spread of clone ST258.
Abstract: The study of six isolates of carbapenem-resistant Klebsiella pneumoniae in patients of a neonatal unit and framed in a surveillance system in hospitals, identified for the first time in our country and South America isolates producing the metallo-β-lactamase type NDM- 1. The purpose of this study was to determinate the molecular and genetic features in these isolates; as well as characterize potential mobilizing structures responsible for the emergence and / or spread of this gene in our country. Molecular analysis showed that all the isolates have the blaNDM-1 gene and gene cassette array aadb-catB3 in a conjugative plasmid ≥ 170 kb of the incompatibility group A / C. Were clonally related by PFGE and multilocus sequence typing showed that all isolates were ST1043, unlike the ST previously reported in NDM, suggesting the emergence of NDM in our country differently from what have found in KPC producing Klebsiella pneumoniae, which is almost entirely due to the spread of clone ST258.