Actividad Antioxidante de extractos de Calendula officinalis asociados a soportes de colágeno tipo I
Autores
Flórez Cabrera, Adriana Matilde
Director
Marín Loaiza, Juan Camilo (Thesis advisor)
Tipo de contenido
Trabajo de grado - Maestría
Idioma del documento
EspañolFecha de publicación
2014-12-01
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Resumen
En este trabajo se evaluó la actividad antioxidante de los metabolitos secundarios presentes en dos extractos, oleoso e hidroglicólico, de flores de Calendula officinalis liberados de forma controlada por soportes de colágeno tipo I con micropartículas de gelatina-colágeno desarrollados mediante ingeniería de tejidos, con el fin determinar su posible aplicación en heridas cutáneas crónicas con elevadas concentraciones de especies reactivas de oxígeno. Se realizaron pruebas de citotoxicidad in vitro siguiendo la norma ISO 10993-5 y la USP 36 (87) para establecer la concentración a la cual los extractos podían ser asociados a los soportes. Para determinar el contenido de compuestos fenólicos se empleó el método Folin-Ciocalteu y para evaluar la actividad antioxidante se emplearon los métodos ORAC (Oxygen radical absorbance capacity) y FRAP (Ferric reducing antioxidant power). A través de las pruebas de citotoxicidad se determinó que concentraciones menores o iguales al 1% v/v y 10% v/v, para extracto hidroglicólico y oleoso respectivamente no son citotóxicas. El mayor contenido de compuestos fenólicos se encontró en el extracto hidroglicólico y la mayor actividad antioxidante en el extracto oleoso, lo cual sugiere que la capacidad antioxidante de los metabolitos secundarios presentes en el extracto oleoso podría ser atribuida a compuestos no fenólicos o a interacciones antagonistas o sinérgicas entre los compuestos fenólicos y otros metabolitos. En conjunto, estos resultados sugieren que el sistema constituido por el soporte de colágeno tipo I con micropartículas de gelatinacolágeno asociado al extracto oleoso, representa una alternativa terapéutica dirigida a modular el estrés oxidativo generado en una herida crónica y por lo tanto a mejorar el proceso de cierre de una herida.
Abstract: In this work tissue engineering scaffolds modified as a control release systems compose of gelatin-collagen microparticle-loaded collagen type I scaffolds were associated to hydroglycolic and oil extracts of Calendula officinalis to improve its bioactivity. The citotoxicity and antioxidant activity was evaluated. To establish the concentration at which the extracts could be associated with the collagen type I scaffolds and to assay the biocompatibility of the collagen scaffold associated with each extracts, the in vitro citotoxicity of each extracts and their association to the scaffold was evaluated according to ISO 10993-5 and USP 36. The total phenolic content was determined by the Follin Ciocalteu method and the antioxidant capacity was detected by FRAP (Ferric reducing antioxidant power) and ORAC (Oxygen radical absorbance capacity) methods. Through citotoxicity assays it was concluded that concentrations less than or equal to 1% v/v and 10% v/v, for hydroglycolic and oil extracts respectively are not citotoxic. The higher content of phenolic compounds was found in the hydroglycolic extract and the highest antioxidant activity assessed through the FRAP and ORAC method was found in the oil extract, suggesting that the antioxidant capacity of secondary metabolites in the oil extract of Calendula officinalis flowers could be attributed to compounds different to polyphenols. Overall, the results suggest that this type of product can be used as an alternative therapy aimed at reducing oxidative stress characteristic of chronic wounds and therefore improve the process of wound healing.
Abstract: In this work tissue engineering scaffolds modified as a control release systems compose of gelatin-collagen microparticle-loaded collagen type I scaffolds were associated to hydroglycolic and oil extracts of Calendula officinalis to improve its bioactivity. The citotoxicity and antioxidant activity was evaluated. To establish the concentration at which the extracts could be associated with the collagen type I scaffolds and to assay the biocompatibility of the collagen scaffold associated with each extracts, the in vitro citotoxicity of each extracts and their association to the scaffold was evaluated according to ISO 10993-5 and USP 36. The total phenolic content was determined by the Follin Ciocalteu method and the antioxidant capacity was detected by FRAP (Ferric reducing antioxidant power) and ORAC (Oxygen radical absorbance capacity) methods. Through citotoxicity assays it was concluded that concentrations less than or equal to 1% v/v and 10% v/v, for hydroglycolic and oil extracts respectively are not citotoxic. The higher content of phenolic compounds was found in the hydroglycolic extract and the highest antioxidant activity assessed through the FRAP and ORAC method was found in the oil extract, suggesting that the antioxidant capacity of secondary metabolites in the oil extract of Calendula officinalis flowers could be attributed to compounds different to polyphenols. Overall, the results suggest that this type of product can be used as an alternative therapy aimed at reducing oxidative stress characteristic of chronic wounds and therefore improve the process of wound healing.