Estudio de la actividad antibiofilm de alquilgliceroles análogos a compuestos de origen marino
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Fernández Montoya, Deicy Julieth
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En el presente estudio se determinó la actividad antibiofilm de 15 Alquilgliceroles (AQG) sintetizados a partir de sus análogos presentes en la naturaleza. En primera instancia se estableció una metodología de ensayo para la generación de biofilm con base en la bibliografía reportada, ajustando el método a las condiciones de trabajo, utilizando como microorganismo de control Pseudomonas aeruginosa ATCC 15442. Se determinó la Concentración Mínima Inhibitoria (CMI) de los AQG utilizando la cepa control y aislados de origen clínico. Con base en la CMI y la metodología establecida de generación de biofilm se evaluó la actividad antibiofilm de los compuestos al 50% y 25% de la CMI de cada AQG frente a los microorganismos de estudio. El grado de inhibición de la producción de biofilm es particular de cada molécula, concentración dependiente y del microorganismo examinado. Los AQG monoinsaturados son más activos que sus homólogos saturados. Las bacterias Gram-positivos son más susceptibles a la acción de los AQG. También se encontraron relaciones estructura función.
Abstract: In the present study the antibiofilm activity of 15 Alkylglycerols (AQG) synthesized from their analogues present in nature was determined. In the first instance, an assay methodology for the generation of biofilm was established based on the reported literature, adjusting the method to working conditions, using as a control microorganism Pseudomonas aeruginosa ATCC 15442. The minimum inhibitory concentration (MIC) of AQG determined, using a control strain and isolates of clinical origin. Based on the MIC and the established methodology of biofilm generation, the antibiofilm activity of the compounds was evaluated at 50% and 25% of the MIC of each one against the microorganisms under study. The degree of inhibition of biofilm depends on each compound, concentration-dependent activity and on the microorganism evaluated. The polyunsatured AQG has little or no activity compared to monounsaturated compounds, which shows mayor activity. Gram positive bacteria are generally more susceptible to AQG action. Structure activity relationships also were found.
Abstract: In the present study the antibiofilm activity of 15 Alkylglycerols (AQG) synthesized from their analogues present in nature was determined. In the first instance, an assay methodology for the generation of biofilm was established based on the reported literature, adjusting the method to working conditions, using as a control microorganism Pseudomonas aeruginosa ATCC 15442. The minimum inhibitory concentration (MIC) of AQG determined, using a control strain and isolates of clinical origin. Based on the MIC and the established methodology of biofilm generation, the antibiofilm activity of the compounds was evaluated at 50% and 25% of the MIC of each one against the microorganisms under study. The degree of inhibition of biofilm depends on each compound, concentration-dependent activity and on the microorganism evaluated. The polyunsatured AQG has little or no activity compared to monounsaturated compounds, which shows mayor activity. Gram positive bacteria are generally more susceptible to AQG action. Structure activity relationships also were found.