Identificación de proteínas de Bacillus thuringiensis con potencial citotóxico
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Rojas Torres, Danithza Sirley
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Trabajo de grado - Maestría
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EspañolFecha de publicación
2019-11-02
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Bacillus thuringiensis se caracteriza por producir proteínas Cry que tienen actividad contra diferentes órdenes de insectos. Se encontró que este microorganismo produce proteínas Cry sin actividad insecticida pero estas nuevas proteinas Cry denominadas parasporinas o proteínas PS tienen actividad citotóxica frente a líneas celulares de cáncer y tienen baja o nula actividad contra células sanas. En este estudio se evaluó 115 cepas nativas de Colombia de Bacillus thuringiensis del cepario de Biopesticidas del Instituto de Biotecnología de la Universidad Nacional que no tenían actividad insecticida para saber si contenían estas proteínas PS. Se encontraron 8 cepas sin actividad hemolítica de las cuales 3 tuvieron una actividad citotóxica frente a las líneas celulares PC-3 (Cáncer de próstata) y MDA -MB231 (Cáncer de mama) considerándolas como posibles proteínas PS con potencial citotóxico, a su vez se recomienda purificar previamente la proteína para aumentar su actividad y secuenciar estas cepas para saber si se trata de una parasporina reportada hasta la fecha.
Abstract: Bacillus thuringiensis is characterized by producing Cry proteins that have activity against different orders of insects. It was found that this microorganism produces Cry proteins without insecticidal activity, these new Cry proteins called parasporins or PS proteins, they have cytotoxic activity against cancer cell lines and have low or no activity against healthy cells. In this study, 115 wild Colombian strains of Bacillus thuringiensis were evaluated from the Biopesticide of the Biotechnology Institute of the National University that did not have insecticidal activity to know if they contained these PS proteins. 8 strains without hemolytic activity were found, of which 3 had a cytotoxic activity against the cell lines PC-3 (prostate cancer) and MDA -MB231 (breast cancer) considering them as possible PS proteins with cytotoxic potential, in turn recommends to previously purify the protein to increase its activity and sequence these strains to know if it is a parasporine reported to date.
Abstract: Bacillus thuringiensis is characterized by producing Cry proteins that have activity against different orders of insects. It was found that this microorganism produces Cry proteins without insecticidal activity, these new Cry proteins called parasporins or PS proteins, they have cytotoxic activity against cancer cell lines and have low or no activity against healthy cells. In this study, 115 wild Colombian strains of Bacillus thuringiensis were evaluated from the Biopesticide of the Biotechnology Institute of the National University that did not have insecticidal activity to know if they contained these PS proteins. 8 strains without hemolytic activity were found, of which 3 had a cytotoxic activity against the cell lines PC-3 (prostate cancer) and MDA -MB231 (breast cancer) considering them as possible PS proteins with cytotoxic potential, in turn recommends to previously purify the protein to increase its activity and sequence these strains to know if it is a parasporine reported to date.