El perfil metabólico se ha usado por debajo de su potencial en la evaluación nutricional y metabólica fundamentalmente por su alto costo que se podría reducir con un pool de muestras de suero de animales bajo las mismas condiciones de manejo. Se trabajó en 210 vacas multíparas durante el periparto sometiendo a las muestras del pool e individuales a iguales condiciones de laboratorio para analizar metabolitos bioquímicos indicadores del metabolismo proteico, energético, mineral y del estatus hepático. Los análisis estadísticos muestran la posibilidad de usar el pool de sueros como alternativa confiable y económica para la realización del perfil metabólico. /Abtrac. The use of metabolic profile test, is currently lesser than its potential power as nutritional and metabolic tool, due mainly to its high cost. Pooled samples could be used to reduce costs. In the work 210 high-yielding dairy cows were used. The laboratory conditions were similar for the two sampling groups. Biochemistries indicators of protein, energy, and mineral metabolism and of hepatic status were analyzed. The statistic analysis included tests for comparison and correlation between pooled samples and individual samples. The use of pooled samples for metabolic profile test may be compared well with individual samples, permitting the use of metabolic profile in a more economic way.