La Guadua angustifolia Kunth, se distribuye en todas las regiones naturales de Colombia, de 51000 ha, 53% se encuentra en el eje cafetero: Quindío, Valle del Cauca, Risaralda y Tolima. El principal uso de la guadua es para construcción, pero también se utiliza en preindustrialización y muebles y artesanías. Se han realizado investigaciones que relacionan la productividad y calidad del culmo con el eco-hábitat, factores hídricos, calidad del suelo, pero no con factores genéticos. Con esta investigación se pretende identificar y genotipificar nueve clones superiores de Guadua angustifolia Kunth, conocer la diversidad genética de la guadua, la estructura poblacional y proponer un modelo para seleccionar clones superiores de G. angustifolia.Para identificar 30 sitios en la zona cafetera que presentaran rodales con guaduas de calidad superior, se realizaron encuestas a aprovechadores, dueños de finca y empresarios y también se revisaron las investigaciones en la zona.//Abstrac: The New World bamboo species, Guadua angustifolia Kunth, is distributed in many geographic regions of Colombia, but is primarily grown in the coffee region departments of Quindío, Valle del Cauca, Risaralda and Tolima, where 53% of the total 51,000 ha are found. The principal uses of this species are in construction but it is also used for furniture and handicraft production but mostly on a non-industrial scale, although processed bamboo products can also be made. G. angustifolia productivity and quality parameters such as culm diameter have been found to be related to ecological parameters, especially hydrology and soil fertility; however, genetic factors have not been studied. Therefore, the objectives of this research project were to evaluate genetic diversity in the species, to describe population structurein various G. angustifoliaforests (known as guaduales) and to identify superior clones of individual plants (known as guaduas).A total of 30 sites with high quality guaduas were identified in the coffee region through surveys conducted with users, harvesters-sellers and growers-owners of guaduales.