Los organismos marinos han sido reportados como una fuente de compuestos biológicamente activos comparados con los aislados de la superficie terrestre. Dentro de los animales marinos las esponjas son una de las más importantes fuentes de nuevos compuestos con diferentes tipos de actividades biológicas. En este estudio, se evaluaron extractos de tres esponjas marinas procedentes del Golfo de Urabá Colombiano con el fin de evaluar sus potenciales genotóxico, citotóxico y antiproliferativo en dos líneas celulares una de hámster (CHO-K1) y otra humana derivada de cáncer (Jurkat). Se analizaron extractos de diferentes polaridades de las esponjas Amphimedon compressa, Iotrochota birotulata y Myrmekioderma gyroderma. Se encontró que el extracto metanólico de la esponja A. compressa mostró un efecto promisorio a una IC50 de 12μg/ml. Adicionalmente se demostró que su efecto estaba relacionado con alteraciones en el ADN además de influir en la progresión del ciclo celular./Abstract.Marine organisms have been reported as a rich source of biologically active compounds, even more than those living on earth. Among marine animals, sponges are one of the most prominent sources of new compounds with different types of biological activities. In this study, we tested extracts from three Colombian marine sponges from the Uraba Golf aiming to evaluate their cytotoxic, genotoxic and anti-proliferative effects on cultured cells lines (hamster CHO-K1 and human leukemia Jurkat cells). We analyzed extracts of different polarities from Amphimedon compressa, Iotrochota birotulata and Myrmekioderma gyroderma sponges. We found that the methanolic extract from A. compressa sponge showed a promissory cytotoxic effect, with an IC50 of 12μg/ml. Additionally, we demonstrate that its cytotoxic effect is related with alterations in DNA and cell cycle progression.Abstract: Marine organisms have been reported as a rich source of biologically active compounds, even more than those living on earth. Among marine animals, sponges are one of the most prominent sources of new compounds with different types of biological activities. In this study, we tested extracts from three Colombian marine sponges from the Uraba Golf aiming to evaluate their cytotoxic, genotoxic and anti-proliferative effects on cultured cells lines (hamster CHO-K1 and human leukemia Jurkat cells). We analyzed extracts of different polarities from Amphimedon compressa, Iotrochota birotulata and Myrmekioderma gyroderma sponges. We found that the methanolic extract from A. compressa sponge showed a promissory cytotoxic effect, with an IC50 of 12µg/ml. Additionally, we demonstrate that its cytotoxic effect is related with alterations in DNA and cell cycle progression.