Ensamblaje del genoma de Leuconostoc mesenteroides IBUN 91.2.98. por medio de herramientas bioinformáticas

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dc.contributor.advisorOspina Sánchez, Sonia Amparo
dc.contributor.authorNúñez Campos, César Augusto
dc.contributor.cvlacNúñez Campos, César Augustospa
dc.contributor.orcidCésar Augusto Núñez Campos [0000000152190836]spa
dc.contributor.researchgroupBiopolímeros y Biofuncionalesspa
dc.date.accessioned2023-04-24T13:54:41Z
dc.date.available2023-04-24T13:54:41Z
dc.date.issued2022-10-07
dc.descriptionilustracionesspa
dc.description.abstractA pesar de la importancia del dextrano en varias aplicaciones industriales y la necesidad de masificar su producción, no se tiene muy documentado el mecanismo de expresión y regulación de las dextransacarasas en cepas productoras como el Leuconostoc mesenteroides cepa IBUN 91.2.98. El desarrollo de métodos de secuenciación de segunda generación y el uso de herramientas bioinformáticas permitirán secuenciar, ensamblar y evaluar genomas completos a un costo relativamente bajo guiados por un genoma de referencia de L. mesenteroides subsp. mesenteroides ATCC 8293, para ayudar al proceso de ensamblaje. La secuenciación produjo un total de 1.47 Gb de datos crudos, que después del trimming y control de calidad generaron 1,40 Gb (7.78 X de profundidad) que se usaron para el ensamblaje. Se obtuvo un ensamblaje para el Leuconostoc mesenteroides cepa IBUN 91.2.98 de una longitud de 2,064 Mpb. La longitud del ensamblaje represento el 85% del tamaño estimado del genoma de referencia de L. mesenteroides subsp. mesenteroides ATCC 8293. (Texto tomado de la fuente)spa
dc.description.abstractDespite the importance of dextran in various industrial applications and the need to massify your production, the mechanism of expression and regulation of dextransucrases in producer strains such as Leuconostoc mesenteroides strain IBUN 91.2.98 has not been well documented. The development of second-generation sequencing methods and the use of bioinformatic tools will make it possible to sequence, assemble, and evaluate complete of genomes at relatively low cost guided by a reference genome of L. mesenteroides subsp. mesenteroides ATCC 8293, to help the assembly process. Sequencing produced a total of 1.47 Gb of raw data, which after trimming and quality control were generated 1.40 Gb (7.78 X deep) which was used for assembly. An assembly for Leuconostoc mesenteroides strain IBUN 91.2.98 with a length of 2,064 Mpb was obtained. The assembly length represented 85% of the estimated size of the reference genome of L. mesenteroides subsp. mesenteroides ATCC 8293.eng
dc.description.degreelevelMaestríaspa
dc.description.degreenameMagíster en Ciencias - Químicaspa
dc.format.extent59 páginasspa
dc.format.mimetypeapplication/pdfspa
dc.identifier.instnameUniversidad Nacional de Colombiaspa
dc.identifier.reponameRepositorio Institucional Universidad Nacional de Colombiaspa
dc.identifier.repourlhttps://repositorio.unal.edu.co/spa
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/83760
dc.language.isospaspa
dc.publisherUniversidad Nacional de Colombiaspa
dc.publisher.branchUniversidad Nacional de Colombia - Sede Bogotáspa
dc.publisher.facultyFacultad de Cienciasspa
dc.publisher.placeBogotá,Colombiaspa
dc.publisher.programBogotá - Ciencias - Maestría en Ciencias - Químicaspa
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dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.licenseReconocimiento 4.0 Internacionalspa
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/spa
dc.subject.ddc000 - Ciencias de la computación, información y obras generalesspa
dc.subject.ddc500 - Ciencias naturales y matemáticasspa
dc.subject.ddc520 - Astronomía y ciencias afinesspa
dc.subject.decsGenomaspa
dc.subject.decsGenomeeng
dc.subject.decsTecnología de bajo costospa
dc.subject.decsLow Cost Technologyeng
dc.subject.proposalreadseng
dc.subject.proposalcontigseng
dc.subject.proposalensamblajespa
dc.subject.proposaltecnologías de secuenciaciónspa
dc.subject.proposaltraducciónspa
dc.titleEnsamblaje del genoma de Leuconostoc mesenteroides IBUN 91.2.98. por medio de herramientas bioinformáticasspa
dc.title.translatedAssembly of the genome of Leuconostoc mesenteroides IBUN 91.2.98. through bioinformatics toolseng
dc.typeTrabajo de grado - Maestríaspa
dc.type.coarhttp://purl.org/coar/resource_type/c_bdccspa
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aaspa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/masterThesisspa
dc.type.redcolhttp://purl.org/redcol/resource_type/TMspa
dc.type.versioninfo:eu-repo/semantics/acceptedVersionspa
dcterms.audience.professionaldevelopmentInvestigadoresspa
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2spa

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