Estudio del microbioma asociado a insectos vectores triatominos en zonas de transmisión de la enfermedad de Chagas

Vista sencilla de ítem
dc.contributor.advisorMoreno Herrera, Claudia Ximena
dc.contributor.authorVarón Saavedra, Angie Natalia
dc.contributor.educationalvalidatorVivero Gomez Rafael Jose
dc.contributor.educationalvalidatorCadavid Restrepo, Gloria Ester
dc.contributor.orcidMoreno Herrera, Claudia Ximena [0000000281325223]
dc.contributor.orcidCadavid Restrepo, Gloria Ester [0000000310266358]
dc.contributor.orcidVivero Gómez, Rafael José [0000000167684097]
dc.contributor.researchgroupMicrobiodiversidad y Bioprospección
dc.date.accessioned2026-02-02T16:36:51Z
dc.date.available2026-02-02T16:36:51Z
dc.date.issued2025
dc.descriptionIlustraciones
dc.description.abstractLa enfermedad de Chagas, causada por Trypanosoma cruzi, continúa siendo un importante problema de salud pública en América Latina; en esta región, la transmisión ocurre principalmente a través de insectos triatominos, que eliminan el parásito en sus heces. Según su capacidad para transmitir el parásito, estos insectos se clasifican en vectores primarios y secundarios En Tolima, Colombia, el vector secundario Rhodnius colombiensis ha adquirido una relevancia epidemiológica debido a su capacidad para colonizar ambientes domiciliarios y a sus altas tasas de infección por T. cruzi, lo que aumenta el riesgo de transmisión en áreas donde los vectores primarios, como R. prolixus, han sido controlados; comprender las interacciones ecoepidemiológicas de este vector, incluyendo sus fuentes de alimentación sanguínea, la microbiota intestinal, la presencia de endosimbiontes y la infección por T. cruzi, es clave para evaluar su capacidad vectorial y su posible uso en estrategias de control biológico. En este estudio se caracterizó el microbioma intestinal de R. colombiensis mediante enfoques dependientes e independientes del cultivo, incluyendo la secuenciación de próxima generación (NGS) del gen 16S rRNA y la detección de endosimbiontes bacterianos, las fuentes de alimentación sanguínea y la presencia de T. cruzi, para esto, se analizaron especímenes en diferentes etapas de desarrollo, provenientes ambientes selváticos e insectario de Coyaima-Tolima y, la identificación de los insectos se realizó mediante claves taxonómicas y análisis molecular del gen COI. Se analizaron 151 especímenes (85 silvestres y 66 de insectario), en el DNA intestinal donde se detectaron los endosimbiontes Cardinium (2,2%) y Microsporidia (4,4%), Didelphis marsupialis y Gallus gallus como fuentes de sangre, y ADN de T. cruzi estuvo presente en el 92% de las muestras evaluadas. El análisis por NGS reveló que los filos bacterianos más abundantes fueron Actinobacteria (54%), Firmicutes (24%) y Proteobacteria (19%), con Gordonia, Lactococcus y Enterobacter como géneros predominantes. Los métodos dependientes del cultivo permitieron aislar y caracterizar 22 cepas bacterianas, siendo Staphylococcus, Yokenella y Dietzia las más representativas. Estos hallazgos sobre la composición y diversidad bacteriana, reportados por primera vez en este estudio, junto con la información sobre sus hospederos silvestres y las tasas de infección por el parásito, destacan la importancia de investigar la microbiota en vectores secundarios como R. colombiensis, este conocimiento puede contribuir a mejorar la vigilancia y el desarrollo de estrategias de control dirigidas a triatominos no domésticos en Colombia. (Texto tomado de la fuente)spa
dc.description.abstractChagas disease, caused by Trypanosoma cruzi, remains a major public health concern in Latin America. In this region, transmission occurs primarily through triatomine insects, which excrete the parasite in their feces. Based on their ability to transmit the parasite, these insects are classified as primary or secondary vectors. In Tolima, Colombia, the secondary vector Rhodnius colombiensis has gained epidemiological relevance due to its ability to colonize domestic environments and its high infection rates with T. cruzi, which increases the risk of transmission in areas where primary vectors, such as R. prolixus, have been controlled. Understanding the eco-epidemiological interactions of this vector— including its blood-feeding sources, gut microbiota, presence of bacterial endosymbionts, and infection with T. cruzi—is key to evaluating its vectorial capacity and potential role in biological control strategies. In this study, the gut microbiome of R. colombiensis was characterized using both culturedependent and culture-independent approaches, including next-generation sequencing (NGS) of the 16S rRNA gene, and the detection of bacterial endosymbionts, blood meal sources, and T. cruzi infection. Specimens at different developmental stages were analyzed, collected from wild habitats and from an insectary in Coyaima, Tolima. Insect identification was carried out using taxonomic keys and molecular analysis of the COI gene. A total of 151 specimens were analyzed (85 wild and 66 from the insectary). In gut DNA, the endosymbionts Cardinium (2.2%) and Microsporidia (4.4%) were detected, as well as Didelphis marsupialis and Gallus gallus as blood meal sources. T. cruzi DNA was present in 92% of the samples tested. NGS analysis revealed that the most abundant bacterial phyla were Actinobacteria (54%), Firmicutes (24%), and Proteobacteria (19%), with Gordonia, Lactococcus, and Enterobacter as the predominant genera. Culture-dependent methods enabled the isolation and characterization of 22 bacterial strains, with Staphylococcus, Yokenella, and Dietzia being the most representative. 4 These findings on the composition and diversity of the bacterial community, reported here for the first time, along with data on wild hosts and parasite infection rates, highlight the importance of studying the microbiota in secondary vectors such as R. colombiensis. This knowledge may contribute to improved surveillance and the development of control strategies targeting non-domestic triatomines in Colombia.eng
dc.description.curricularareaBiotecnología.Sede Medellín
dc.description.degreelevelMaestría
dc.description.degreenameMagíster en Ciencias - Biotecnología
dc.description.researchareaEcología Microbiana
dc.format.extent1 recurso en líne (127 páginas)
dc.format.mimetypeapplication/pdf
dc.identifier.instnameUniversidad Nacional de Colombiaspa
dc.identifier.reponameRepositorio Institucional Universidad Nacional de Colombiaspa
dc.identifier.repourlhttps://repositorio.unal.edu.co/spa
dc.identifier.urihttps://repositorio.unal.edu.co/handle/unal/89358
dc.language.isospa
dc.publisherUniversidad Nacional de Colombia
dc.publisher.branchUniversidad Nacional de Colombia - Sede Medellín
dc.publisher.facultyFacultad de Ciencias
dc.publisher.placeMedellín, Colombia
dc.publisher.programMedellín - Ciencias - Maestría en Ciencias - Biotecnología
dc.relation.referencesAbad-Franch, F., Monteiro, F. A., Jaramillo, N. O., Gurgel-Gonçalves, R., Dias, F. B. S., & Diotaiuti, L. (2009). Ecology, evolution, and the long-term surveillance of vector-borne Chagas disease: A multi-scale appraisal of the tribe Rhodniini (Triatominae). Acta Tropica, 110(2-3), 159–177. https://doi.org/10.1016/j.actatropica.2008.06.005
dc.relation.referencesAbras, A., Ballart, C., Fernández-Arévalo, A., Pinazo, M. J., Gascón, J., Muñoz, C., & Gállego, M. (2022). Worldwide control and management of Chagas disease in a new era of globalization: A close look at congenital Trypanosoma cruzi infection. Clinical Microbiology Reviews, 35(2), e0015221. https://doi.org/10.1128/cmr.00152-21
dc.relation.referencesAchari, G. A., & Ramesh, R. (2015). Characterization of bacteria degrading 3-hydroxy palmitic acid methyl ester (3OH-PAME), a quorum sensing molecule of Ralstonia solanacearum. Letters in Applied Microbiology, 60(5), 447–455. https://doi.org/10.1111/lam.12389
dc.relation.referencesAllen, K., & Lineberry, M. (2022). Trypanosoma cruzi and other vector-borne infections in shelter dogs in two counties of Oklahoma, United States. Vector-Borne and Zoonotic Diseases, 22(5), 273–279. https://doi.org/10.1089/VBZ.2021.0078
dc.relation.referencesAlves, B., & Gonçalves, D. (2015). Neglected diseases and bioethics: Dialogue between an old problem and a new area of knowledge. Revista Bioética, 23(1), 104–116. https://doi.org/10.1590/1983-80422015231051
dc.relation.referencesAraujo, T., Telleria, J., & Dalenz, J. (2017). History of the discovery of the American trypanosomiasis (Chagas disease). In J. Telleria & M. Tibayrenc (Eds.), American Trypanosomiasis Chagas Disease One Hundred Years of Research (2nd ed., pp. 1-22). Elsevier.
dc.relation.referencesArias-Giraldo, L. M., Muñoz, M., Hernández, C., Herrera, G., Velásquez-Ortiz, N., Cantillo-Barraza, O., Urbano, P., Cuervo, A., & Ramírez, J. (2020). Identification of blood-feeding sources in Panstrongylus, Psammolestes, Rhodnius, and Triatoma using amplicon-based next-generation sequencing. Parasites & Vectors, 13, 434. https://doi.org/10.1186/s13071-020-04310-z
dc.relation.referencesAzambuja, P., Feder, D., & Garcia, E. (2004). Isolation of Serratia marcescens in the midgut of Rhodnius prolixus: Impact on the establishment of the parasite Trypanosoma cruzi in the vector. Experimental Parasitology, 107(1–2), 89-96. https://doi.org/10.1016/j.exppara.2004.06.002
dc.relation.referencesAzambuja, P., Garcia, E. S., & Ratcliffe, N. A. (2005). Gut microbiota and parasite transmission by insect vectors. Trends in Parasitology, 21(12), 568-572. https://doi.org/10.1016/j.pt.2005.09.011
dc.relation.referencesBarbosa, H. J., Quevedo, Y. S., Torres, A. M., Veloza, G. A. G., Carranza Martínez, J. C., Urrea-Montes, D. A., et al. (2024). Análisis proteómico comparativo de la hemolinfa y las glándulas salivales de Rhodnius prolixus y R. colombiensis revela candidatos asociados con actividad lítica diferencial frente a Trypanosoma cruzi Dm28c y T. cruzi Y. PLOS Neglected Tropical Diseases, 18(4), e0011452. https://doi.org/10.1371/journal.pntd.0011452
dc.relation.referencesBasset, Y., Cizek, L., Cuénoud, P., Didham, R. K., Guilhaumon, F., Missa, O., Novotny, V., Ødegaard, F., Roslin, T., & Leponce, M. (2012). Arthropod diversity in a tropical forest. Science, 338(6113), 1481–1484. https://doi.org/10.1126/science.1226727
dc.relation.referencesBatista, K., Vieira, C., Figueiredo, M., Costa, S., Azambuja, P., Genta, F., & Castro, D. (2021). Influence of Serratia marcescens and Rhodococcus rhodnii on the humoral immunity of Rhodnius prolixus. International Journal of Molecular Sciences, 22(20), 10901. https://doi.org/10.3390/ijms222010901
dc.relation.referencesBeard, C. B., Dotson, E. M., Pennington, P. M., Eichler, S., Cordon-Rosales, C., & Durvasula, R. V. (2001). Bacterial symbiosis and paratransgenic control of vectorborne Chagas disease. International Journal for Parasitology, 31(5–6), 621–627. https://doi.org/10.1016/S0020-7519(01)00165-5
dc.relation.referencesBranda, F., Cella, E., Scarpa, F., Slavov, S. N., Bevivino, A., Moretti, R., Degafu, A. L., Pecchia, L., Rizzo, A., Defilippo, F., Moreno, A., Ceccarelli, G., Alcantara, L. C. J., Ferreira, A., Ciccozzi, M., & Giovanetti, M. (2024). Wolbachia-based approaches to controlling mosquito-borne viral threats: Innovations, AI integration, and future directions in the context of climate change. Viruses, 16(12), 1868. https://doi.org/10.3390/v16121868
dc.relation.referencesBrenière, S. F., Waleckx, E., & Barnabé, C. (2016). Over six thousand Trypanosoma cruzi strains classified into discrete typing units (DTUs): Attempt at an inventory. PLOS Neglected Tropical Diseases, 10(8), e0004792. https://doi.org/10.1371/journal.pntd.0004792
dc.relation.referencesBrown, J. J., Rodríguez-Ruano, S. M., Poosakkannu, A., Batani, G., Schmidt, J. O., Roachell, W., Zima, J., Hypša, V., & Nováková, E. (2020). Ontogeny, species identity, and environment dominate microbiome dynamics in wild populations of kissing bugs (Triatominae). Microbiome, 8(1), 1–16. https://doi.org/10.1186/S40168-020-00921-X
dc.relation.referencesBukhari, T., Pevsner, R., & Herren, J. K. (2022). Microsporidia: A promising vector control tool for residual malaria transmission. Frontiers in Tropical Diseases, 3, 957109. https://doi.org/10.3389/fitd.2022.957109
dc.relation.referencesCabrera Orrego, R., Espinosa Muñoz, D. Y., Durango Manrique, Y., Mendoza Uribe, W. L., Gómez García, G. F., & Gutiérrez Builes, L. A. (2021). Enfermedades transmitidas por vectores. Universidad Pontificia Bolivariana. http://hdl.handle.net/20.500.11912/8619
dc.relation.referencesCambronero-Heinrichs, J. C., Rojas-Gätjens, D., Baizán, M., Alvarado-Ocampo, J., Rojas-Jimenez, K., Loaiza, R., Chavarría, M., Calderón-Arguedas, Ó., & Troyo, A. (2024). Highly abundant bacteria in the gut of Triatoma dimidiata (Hemiptera: Reduviidae) can inhibit the growth of Trypanosoma cruzi (Kinetoplastea: Trypanosomatidae). Journal of Medical Entomology, 61(6), 1333–1344. https://doi.org/10.1093/jme/tjae012
dc.relation.referencesCantillo-Barraza, O., Bedoya, S. C., Xavier, S. C. C., Zuluaga, S., Salazar, B., Vélez-Mira, A., Carrillo, L. M., & Triana-Chávez, O. (2020). Trypanosoma cruzi infection in domestic and synanthropic mammals as a potential risk of sylvatic transmission in a rural area from north of Antioquia, Colombia. Parasite Epidemiology and Control, 11, e00171. https://doi.org/10.1016/j.parepi.2020.e00171
dc.relation.referencesCastillo, Méndez & Ahumada. (2024). Clave pictórica para formas adultas de los vectores de la enfermedad de Chagas (Hemiptera: Reduviidae: Triatominae) de Colombia. Instituto Nacional de Salud. https://www.ins.gov.co/BibliotecaDigital/clave-pictorica-para-formas-adultas-de-los-vectores-de-la-enfermedad-de-chagas-hemiptera-reduviidae-triatominae-de-colombia.pdf
dc.relation.referencesCastro, D. P., Seabra, S. H., García, E. S., de Souza, W., & Azambuja, P. (2007). Trypanosoma cruzi: Estudios ultraestructurales de adhesión, lisis y formación de biofilm por Serratia marcescens. Parasitología Experimental, 117(2), 201–207. https://doi.org/10.1016/j.exppara.2007.04.014
dc.relation.referencesCavalier-Smith, T. (2009). Predation and eukaryote cell origins: A coevolutionary perspective. The International Journal of Biochemistry & Cell Biology, 41(2), 307–322. https://doi.org/10.1016/j.biocel.2008.10.002
dc.relation.referencesCDC. (2024). Centers for Disease Control and Prevention. American Trypanosomiasis. U.S. Department of Health & Human Services. Retrieved November 20, 2024, from https://www.cdc.gov/dpdx/trypanosomiasisamerican/index.html
dc.relation.referencesChala, B., & Hamde, F. (2021). Emerging and re-emerging vector-borne infectious diseases and the challenges for control: A review. Frontiers in Public Health, 9, 715759. https://doi.org/10.3389/fpubh.2021.715759
dc.relation.referencesChaves, L. F., Friberg, M. D., Pascual, M., Calzada, J. E., Luckhart, S., & Bergmann, L. R. (2024). Investigación al servicio de la comunidad que aborda los impactos del cambio climático en las enfermedades transmitidas por vectores. Vista Personal, 8(5), e334-e341.
dc.relation.referencesCirimotich, C., Ramirez, J., Dimopoulos, G., & Feinstone, W. (2011). Native microbiota shape insect vector competence for human pathogens. Cell Host & Microbe, 10(6), 484–494. https://doi.org/10.1016/j.chom.2011.09.006
dc.relation.referencesClear, R., Dumonteil, E., & Herrera, C. (2025). Decoding Chagas disease: What next-generation sequencing has taught us. En Recent Advances in Parasitomics (pp. 43–63). Springer Nature.
dc.relation.referencesCox, J. W., Ballweg, R. A., Taft, D. H., Velayutham, P., Haslam, D. B., & Porollo, A. (2017). A fast and robust protocol for metataxonomic analysis using RNAseq data. Microbiome, 5, Article 7. https://doi.org/10.1186/s40168-017-0235-2
dc.relation.referencesCruz, J. (2022). Arritmias cardiacas malignas y muerte súbita cardiaca en la enfermedad de Chagas. Una revisión bibliográfica. Universidad Católica de Cuenca. https://dspace.ucacue.edu.ec/handle/ucacue/11853
dc.relation.referencesCucunubá, Z. M., Gutiérrez-Romero, S. A., Ramírez, J.-D., Velásquez-Ortiz, N., Ceccarelli, S., Parra-Henao, G., Henao-Martínez, A. F., Rabinovich, J., Basáñez, M.-G., Nouvelle, P., & Abad-Franchi, F. (2024). Epidemiología de la enfermedad de Chagas en las Américas. Serie, 3710(881).
dc.relation.referencesde Lana, M., & de Menezes Machado, E. M. (2017). Biology of Trypanosoma cruzi and biological diversity. En American Trypanosomiasis Chagas Disease (Second Edition): One Hundred Years of Research (pp. 345–369). https://doi.org/10.1016/B978-0-12-801029-7.00016-2
dc.relation.referencesde Souza, W. M., & Weaver, S. C. (2024). Effects of climate change and human activities on vector-borne diseases. Nature Reviews Microbiology, 22, 476–491. https://doi.org/10.1038/s41579-024-01026-0
dc.relation.referencesDíaz, M. L., & González, C. I. (2014). Enfermedad de Chagas agudo: Transmisión oral de Trypanosoma cruzi como una vía de transmisión re-emergente. Revista de la Universidad Industrial de Santander. Salud, 46(2), 177-188. http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0121-08072014000200009
dc.relation.referencesDíaz, S., Villavicencio, B., Correia, N. Costa, & Haag, K. (2016). Triatomine bugs, their microbiota, and Trypanosoma cruzi: Asymmetric responses of bacteria to an infected blood meal. Parasites & Vectors, 9, 636. https://doi.org/10.1186/s13071-016-1926-2
dc.relation.referencesDillon, R. J., & Dillon, V. M. (2004). The gut bacteria of insects: Nonpathogenic interactions. Annual Review of Entomology, 49, 71–92. https://doi.org/10.1146/annurev.ento.49.061802.123416
dc.relation.referencesDuguma, D., Hall, M. W., Smartt, C. T., & Neufeld, J. D. (2017). Temporal variations of microbiota associated with the immature stages of two Florida Culex mosquito vectors. Microbial Ecology, 74(4), 979–989. https://doi.org/10.1007/s00248-017-0988-9
dc.relation.referencesDujardin, J.-P., Schofield, J., & Panzera, F. (2002). Los vectores de la enfermedad de Chagas. Académie Royale des Sciences d'Outre-Mer.
dc.relation.referencesDumonteil, E., Pronovost, H., Bierman, E. F., Sanford, A., Majeau, A., Moore, R., & Herrera, C. (2020). Interactions among Triatoma sanguisuga blood feeding sources, gut microbiota, and Trypanosoma cruzi diversity in southern Louisiana. Molecular Ecology, 29(19), 3747–3761. https://doi.org/10.1111/mec.15582
dc.relation.referencesDuron, O., Bouchon, D., Boutin, S., Bellamy, L., Zhou, L., Engelstädter, J., & Hurst, G. D. D. (2008). The diversity of reproductive parasites among arthropods: Wolbachia do not walk alone. BMC Biology, 6(1), 27. https://doi.org/10.1186/1741-7007-6-27
dc.relation.referencesDurvasula, R. V., Gumbs, A., Panackal, A., Kruglov, O., Aksoy, S., Merrifield, R. B., Richards, F. F., & Beard, C. B. (1997). Prevention of insect-borne disease: An approach using transgenic symbiotic bacteria. Proceedings of the National Academy of Sciences, 94(7), 3274–3278. https://doi.org/10.1073
dc.relation.referencesEberhard, F. E., Klimpel, S., Guarneri, A. A., & Tobias, N. J. (2022). Exposure to Trypanosoma parasites induces changes in the microbiome of the Chagas disease vector Rhodnius prolixus. Microbiome, 10(1), 45. https://doi.org/10.1186/s40168-022-01240-z
dc.relation.referencesEdgar, R. C. (2004). MUSCLE: Multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research, 32(5), 1792–1797. https://doi.org/10.1093/nar/gkh340
dc.relation.referencesEichler, S., & Schaub, G. A. (2002). Development of symbionts in triatomine bugs and the effects of infections with trypanosomatids. Experimental Parasitology, 100(1), 17–27. https://doi.org/10.1006/expr.2001.4653
dc.relation.referencesEleftherianos, I., Atri, J., Accetta, J., & Castillo, J. C. (2013). Endosymbiotic bacteria in insects: Guardians of the immune system? Frontiers in Physiology, 4, 46. https://doi.org/10.3389/fphys.2013.00046
dc.relation.referencesEngel, P., & Moran, N. (2013). The gut microbiota of insects – diversity in structure and function. FEMS Microbiology Reviews, 37(5), 699–735. https://doi.org/10.1111/1574-6976.12025
dc.relation.referencesEspejo, R., Feijóo, C. G., Romerol, J., & Vásquez, M. (1998). PACE analysis of the heteroduplexes formed between PCR-amplified 16S rRNA genes: Estimation of sequence similarity and rDNA complexity.
dc.relation.referencesEspino, C., Gómez, T., González, G., Brazil Do Santos, M., Solano, J., Sousa, O., Moreno, N., Windsor, D., Ying, A., Vilchez, S., & Osuna, A. (2009). Detection of Wolbachia bacteria in multiple organs and feces of the triatomine insect Rhodnius pallescens (Hemiptera, Reduviidae). Applied and Environmental Microbiology, 75(2), 547–550. https://doi.org/10.1128/AEM.01665-08
dc.relation.referencesFaria da Mota, F., Pinheiro Marinho, L., José de Carvalho Moreira, C., Lima, M. M., Mello, C. B., Souza Garcia, E., Carels, N., & Azambuja, P. (2012). Cultivation-independent methods reveal differences among bacterial gut microbiota in triatomine vectors of Chagas disease. PLOS Neglected Tropical Diseases, 6(8), e1631. https://doi.org/10.1371/journal.pntd.0001631
dc.relation.referencesFeldhaar, H. (2011). Bacterial symbionts as mediators of ecologically important traits of insect hosts. Ecological Entomology, 36(5), 533–543. https://doi.org/10.1111/j.1365-2311.2011.01318.x
dc.relation.referencesFerrarini, M. G., Agnès, V., Vincent-Monégat, C., Dellnyaglio, E., Gillet, B., Hughes, S., Hurtado, O., Condemine, G., Zaidman-Rémy, A., Rebollo, R., Parisot, N., & Heddi, A. (2023). La coordinación de la expresión génica del huésped y del endosimbionte rige el crecimiento y la eliminación del endosimbionte en el gorgojo del cereal Sitophilus spp. Microbiome, 11, 274. https://doi.org/10.1186/s40168-023-01714-8
dc.relation.referencesFieck, A., Hurwitz, I., Kang, A. S., & Durvasula, R. (2010). Trypanosoma cruzi: Synergistic cytotoxicity of multiple amphipathic antimicrobial peptides to T. cruzi and potential bacterial hosts. Experimental Parasitology, 125(4), 342–347. https://doi.org/10.1016/j.exppara.2010.02.016
dc.relation.referencesFlores-Ferrer, A., Marcou, O., Waleckx, E., Dumonteil, E., & Gourbière, S. (2017). Evolutionary ecology of Chagas disease; What do we know and what do we need? Evolutionary Applications, 11(4), 470–487. https://doi.org/10.1111/eva.12582
dc.relation.referencesFolmer, O., Black, M., Hoeh, W., Lutz, R., & Vrijenhoek, R. (1994). DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3(5), 294-299.
dc.relation.referencesGalvão, C., & Justi, S. A. (2015). An overview on the ecology of Triatominae (Hemiptera: Reduviidae). Acta Tropica, 151, 116–125. https://doi.org/10.1016/j.actatropica.2015.06.006
dc.relation.referencesGeospiza, Inc. (2015). FinchTV 1.4.0 [Software]. Disponible en: https://digitalworldbiology.com/FinchTV
dc.relation.referencesGhosh, K., & Weiss, L. M. (2009). Molecular diagnostic tests for microsporidia. Interdisciplinary Perspectives on Infectious Diseases, 2009, Article ID 926521. https://doi.org/10.1155/2009/926521
dc.relation.referencesGilliland, C. A., & Vogel, K. J. (2025). Gut bacteria induce heterologous immune priming in Rhodnius prolixus encompassing both humoral and cellular immune responses. bioRxiv. https://doi.org/10.1101/2025.01.31.635857
dc.relation.referencesGrijalva, M. J., Villacís, A. G., Moncayo, A. L., & Ocaña-Mayorga, S. (2017). Distribution of triatomine species in domestic and peridomestic environments in central coastal Ecuador. PLOS Neglected Tropical Diseases, 11(10), e0005970. https://doi.org/10.1371/journal.pntd.0005970
dc.relation.referencesGuhl, F. (2009). Enfermedad de Chagas: Realidad y perspectivas. Revista Biomédica, 20(3), 228–234. https://www.revistabiomedica.mx/index.php/revbiomed/article/view/139
dc.relation.referencesGuhl, F., & Ramírez, J. D. (2013). Retrospective molecular integrated epidemiology of Chagas disease in Colombia. Infection, Genetics and Evolution, 20, 148–154. https://doi.org/10.1016/j.meegid.2013.08.028
dc.relation.referencesGuhl, F., Aguilera, G., Pinto, N., & Vergara, D. (2007). Actualización de la distribución geográfica y ecoepidemiología de la fauna de triatominos (Reduviidae: Triatominae) en Colombia. Biomédica, 27(Suppl. 1), 143-162. Retrieved March 19, 2023, from http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-41572007000500016&lng=en&tlng=es
dc.relation.referencesGupta, A., & Nair, S. (2020). Dynamics of insect-microbiome interaction influence host and microbial symbiont. Frontiers in Microbiology, 11, 1357. https://doi.org/10.3389/fmicb.2020.01357
dc.relation.referencesGürtler, R. E., & Diotaiuti, L. (2017). Triatominae. In J. Telleria & M. Tibayrenc (Eds.), American Trypanosomiasis Chagas Disease (2nd ed., pp. 287-315). Elsevier. https://doi.org/10.1016/B978-0-12-801029-7.00014-9
dc.relation.referencesGurung, K., Wertheim, B., & Falcao Salles, J. (2019). The microbiome of pest insects: It is not just bacteria. Entomologia Experimentalis et Applicata, 167(3), 156–170. https://doi.org/10.1111/EEA.12768
dc.relation.referencesHamilton, P., & Stevens, J. R. (2017). Classification and phylogeny of Trypanosoma cruzi. En American Trypanosomiasis Chagas Disease (pp. 321–344). https://doi.org/10.1016/B978-0-12-801029-7.00015-7
dc.relation.referencesHammer, T., Janzen, D., Hallwachs, W., Jaffe, S., & Fierer, N. (2017). Caterpillars lack a resident gut microbiome. Proceedings of the National Academy of Sciences of the United States of America, 114(36), 9641–9646. https://doi.org/10.1073/PNAS.1707186114
dc.relation.referencesHebert, P. D., Cywinska, A., Ball, S. L., & deWaard, J. R. (2003). Biological identifications through DNA barcodes. Proceedings. Biological Sciences, 270(1512), 313–321. https://doi.org/10.1098/rspb.2002.2218
dc.relation.referencesHernández, C., da Rosa, J., Vallejo, G., Guhl, F., & Ramírez, J. (2020). Taxonomy, evolution, and biogeography of the Rhodniini tribe (Hemiptera: Reduviidae). Diversity, 12(3), 97. https://doi.org/10.3390/D12030097
dc.relation.referencesHerrera, L. (2010). Una revisión sobre reservorios de Trypanosoma (Schizotrypanum) cruzi (Chagas, 1909), agente etiológico de la enfermedad de Chagas. Boletín de Malariología y Salud Ambiental, 50(1), 3-15. Recuperado el 10 de febrero de 2025, de http://ve.scielo.org/scielo.php?script=sci_arttext&pid=S1690-46482010000100002
dc.relation.referencesHoffmann, A. A., & Cooper, B. S. (2024). Describing endosymbiont–host interactions within the parasitism–mutualism continuum. Ecology and Evolution, 14(7), e11705. https://doi.org/10.1002/ece3.11705
dc.relation.referencesHu, Y., Xie, H., Gao, M., Huang, P., Zhou, H., Ma, Y., Zhou, M., Liang, J., Yang, J., & Lv, Z. (2020). Dynamic of composition and diversity of gut microbiota in Triatoma rubrofasciata in different developmental stages and environmental conditions. Frontiers in Cellular and Infection Microbiology, 10, 587708. https://doi.org/10.3389/fcimb.2020.587708
dc.relation.referencesHuang, S. K., Ye, K. T., Huang, W. F., Ying, B. H., Su, X., Lin, L. H., Li, J. H., Chen, Y. P., Li, J. L., Bao, X. L., & Hu, J. Z. (2018). Influence of feeding type and Nosema ceranae infection on the gut microbiota of Apis cerana workers. mSystems, 3(6), e00177-18. https://doi.org/10.1128/mSystems.00177-18
dc.relation.referencesHuerta-García, A., & Álvarez-Cervantes, J. (2024). The gut microbiota of insects: A potential source of bacteria and metabolites. International Journal of Tropical Insect Science, 44, 13–30. https://doi.org/10.1007/s42690-023-01147-8
dc.relation.referencesJamovi Project. (2018). Jamovi (Versión 0.9) [Computer Software].
dc.relation.referencesJansen, A. M., Roque, A. L. R., & Xavier, S. (2017). Ciclo enzoótico de Trypanosoma cruzi: Aspectos generales, huéspedes y reservorios domésticos y sinantrópicos. En Telleria, J., & Tibayrenc, M. (Eds.), Tripanosomiasis Americana. Enfermedad de Chagas. Cien Años de Investigación (2a ed.). Prensa Académica.
dc.relation.referencesJansen, A. M., Xavier, S. C. D. C., & Roque, A. L. R. (2018). Trypanosoma cruzi transmission in the wild and its most important reservoir hosts in Brazil. Parasites & Vectors, 11(1), 502. https://doi.org/10.1186/s13071-018-3067-2
dc.relation.referencesJansen, A. M., Xavier, S. C., & Roque, A. L. (2015). The multiple and complex and changeable scenarios of the Trypanosoma cruzi transmission cycle in the sylvatic environment. Acta Tropica, 151, 1–15. https://doi.org/10.1016/j.actatropica.2015.07.018
dc.relation.referencesJensen, M., Webster, J. A., & Straus, N. (1993). Rapid identification of bacteria on the basis of polymerase chain reaction-amplified ribosomal DNA spacer polymorphisms. Applied and Environmental Microbiology, 59(4), 945–952.
dc.relation.referencesJing, T. Z., Qi, F. H., & Wang, Z. Y. (2020). Most dominant roles of insect gut bacteria: Digestion, detoxification, or essential nutrient provision? Microbiome, 8(1), 38. https://doi.org/10.1186/s40168-020-00823-y
dc.relation.referencesJing, Y., Wang, Q., Bai, F., Li, Z., Li, Y., Liu, W., Yan, Y., Zhang, S., Gao, C., & Yu, Y. (2024). Role of microbiota-gut-brain axis in natural aging-related alterations in behavior. Frontiers in Neuroscience, 18, 1362239. https://doi.org/10.3389/fnins.2024.1362239
dc.relation.referencesJurberg, J., & Galvão, C. (2006). Biology, ecology and systematics of Triatominae (Heteroptera, Reduviidae), vectors of Chagas disease, and implications for human health. [Artículo publicado en ResearchGate]. https://www.researchgate.net/publication/237153374
dc.relation.referencesJusti, S. A., Russo, C. A. M., dos Santos Mallet, J. R., Obara, M. T., & Galvão, C. (2014). Molecular phylogeny of Triatomini (Hemiptera: Reduviidae: Triatominae). Parasites & Vectors, 7, 149. https://doi.org/10.1186/1756-3305-7-149
dc.relation.referencesJusti, S., & Galvão, C. (2017). The evolutionary origin of diversity in Chagas disease vectors. Trends in Parasitology, 33(1), 11. https://doi.org/10.1016/j.pt.2016.11.002
dc.relation.referencesJusti, S., Galvão, C., & Schrago, G. (2016). Geological changes of the Americas and their influence on the diversification of the neotropical kissing bugs (Hemiptera: Reduviidae: Triatominae). PLoS Neglected Tropical Diseases, 10, e0004527. https://doi.org/10.1371/journal.pntd.0004527
dc.relation.referencesKassem, H. A., & Osaman, G. (2007). Transmisión materna de Wolbachia en Phlebotomus papatasi (Scopoli). Annals of Tropical Medicine & Parasitology, 101, 435–440.
dc.relation.referencesKitano, T., Umetsu, K., Tian, W., & Osawa, M. (2007). Two universal primer sets for species identification among vertebrates. International Journal of Legal Medicine, 121(5), 423-427. https://doi.org/10.1007/s00414-006-0113-x
dc.relation.referencesKlotz, S. A., Dorn, P. L., & Schmidt, J. O. (2014). Kissing bugs in the United States: Risk for vector-borne disease in humans. Environmental Health Insights, 8, 49–59. https://doi.org/10.4137/EHI.S16003
dc.relation.referencesKocher, T. D., Thomas, W. K., Meyer, A., Edwards, S. V., Pääbo, S., Villablanca, F. X., & Wilson, A. C. (1989). Dynamics of mitochondrial DNA evolution in animals: Amplification and sequencing with conserved primers. Proceedings of the National Academy of Sciences, 86(16), 6196-6200. https://doi.org/10.1073/pnas.86.16.6196
dc.relation.referencesKopecky, J., Perotti, M. A., Nesvorna, M., Erban, T., & Hubert, J. (2013). Cardinium endosymbionts are widespread in synanthropic mite species (Acari: Astigmata). Journal of Invertebrate Pathology, 112(1), 20–23. https://doi.org/10.1016/j.jip.2012.11.001
dc.relation.referencesKrams, I. A., Kecko, S., Jõers, P., Trakimas, G., Elferts, D., Krams, R., Luoto, S., Rantala, M. J., Inashkina, I., Gudrā, D., Fridmanis, D., Contreras-Garduño, J., Grantiņa-Ieviņa, L., & Krama, T. (2017). Microbiome symbionts and diet diversity incur costs on the immune system of insect larvae. The Journal of Experimental Biology, 220(18), 3221–3229. https://doi.org/10.1242/jeb.161232
dc.relation.referencesLaboratorio de Investigaciones en Parasitología Tropical. (s.f.). Método de captura de triatominos. Universidad del Tolima. Recuperado el 11 de febrero de 2025, de http://investigaciones.ut.edu.co/22-grupos-de-investigacion/85-laboratorio-de-investigaciones-en-parasitologia-tropical.html
dc.relation.referencesLazzari, C. R., Fauquet, A., & Lahondère, C. (2018). Keeping cool: Kissing bugs avoid cannibalism by thermoregulating. Journal of Insect Physiology, 107, 29–33. https://doi.org/10.1016/j.jinsphys.2018.02.006
dc.relation.referencesLee, J.-H., Lee, K.-A., & Lee, W.-J. (2017). Microbiota, gut physiology, and insect immunity. En Advances in Insect Physiology (Vol. 52, pp. 111-138). Elsevier. https://doi.org/10.1016/bs.aiip.2016.11.001
dc.relation.referencesLewis, Z., & Lizé, A. (2015). Insect behaviour and the microbiome. Current Opinion in Insect Science, 9, 86–90. https://doi.org/10.1016/j.cois.2015.03.003
dc.relation.referencesLin, D., Zheng, X., Sanogo, B., Ding, T., Sun, X., & Wu, Z. (2021). Bacterial composition of midgut and entire body of laboratory colonies of Aedes aegypti and Aedes albopictus from Southern China. Parasites & Vectors, 14, Article 586. https://doi.org/10.1186/s13071-021-05076-7
dc.relation.referencesLorenzo, M. G., Lazzari, C. R., & Barrozo, R. B. (2025). Beyond blood: The flexibility of triatomine bug food search and recognition. Current Opinion in Insect Science, 68, 101301. https://doi.org/10.1016/j.cois.2024.101301 Magalhães, L. M. D., Gollob, K. J., Zingales, B., & Dutra, W. O. (2021). Pathogen diversity, immunity, and the fate of infections: Lessons learned from Trypanosoma cruzi human–host interactions. The Lancet Microbe, 2(12), e646–e658. https://doi.org/10.1016/S2666-5247(21)00265-2
dc.relation.referencesMaitre, A., Wu-Chuang, A., Aželytė, J., Palinauskas, V., Mateos-Hernández, L., Obregon, D., Hodžić, A., Valiente Moro, C., Estrada-Peña, A., Paoli, J. C., Falchi, A., & Cabezas-Cruz, A. (2022). Vector microbiota manipulation by host antibodies: The forgotten strategy to develop transmission-blocking vaccines. Parasites & Vectors, 15(1), 4. https://doi.org/10.1186/s13071-021-05122-5
dc.relation.referencesMann, A. E., Mitchell, E. A., Zhang, Y., Curtis-Robles, R., Thapa, S., Hamer, S. A., & Allen, M. S. (2020). Comparison of the bacterial gut microbiome of North American Triatoma spp. with and without Trypanosoma cruzi. Frontiers in Microbiology, 11, 364. https://doi.org/10.3389/fmicb.2020.00364
dc.relation.referencesMarcet, P. L., Mora, M. S., Cutrera, A. P., Jones, L., Gürtler, R. E., Kitron, U., & Dotson, E. M. (2008). Genetic structure of Triatoma infestans populations in rural communities of Santiago del Estero, northern Argentina. Infection, Genetics and Evolution, 8(6), 835–846. https://doi.org/10.1016/j.meegid.2008.08.002
dc.relation.referencesMarchesi, J. R., & Ravel, J. (2015). The vocabulary of microbiome research: a proposal. Microbiome, 3, Article 31. https://doi.org/10.1186/s40168-015-0094-5
dc.relation.referencesMarti, G. A., Balsalobre, A., Susevich, M. L., Rabinovich, J. E., & Echeverría, M. G. (2015). Detection of triatomine infection by Triatoma virus and horizontal transmission: Protecting insectaries and prospects for biological control. Journal of Invertebrate Pathology, 124, 57–60. https://doi.org/10.1016/j.jip.2014.10.008
dc.relation.referencesMarulanda-Moreno, S. M., Saldamando-Benjumea, C. I., Vivero Gomez, R., Cadavid-Restrepo, G., & Moreno-Herrera, C. X. (2024). Comparative analysis of Spodoptera frugiperda (J. E. Smith) (Lepidoptera, Noctuidae) corn and rice strains microbiota revealed minor changes across life cycle and strain endosymbiont association. PeerJ, 12, e17087. https://doi.org/10.7717/peerj.17087
dc.relation.referencesMechan, F., Bartonicek, Z., Malone, D., & Romero, S. (2023). Unmanned aerial vehicles for surveillance and control of vectors of malaria and other vector-borne diseases. Malaria Journal, 22, 23. https://doi.org/10.1186/s12936-022-04414-0
dc.relation.referencesMejía, A. M., & Triana, O. (2005). Análisis por LSSP-PCR de la variabilidad genética de Trypanosoma cruzi en sangre y órganos de ratones. Biomédica, 25(1), 76-86. Recuperado en 20 de febrero de 2025, de http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-41572005000100009
dc.relation.referencesMeneses, A., Rodríguez, C., Suárez, Y., Carranza, J., & Vallejo, G. (2020). Salivary proteins electrophoretic patterns enabled differentiating Colombian Rhodnius Trans-Andean and Cis-Andean groups. Biomedica: Revista del Instituto Nacional de Salud, 40(2), 404–411. https://doi.org/10.7705/biomedica.4992
dc.relation.referencesMereghetti, V., Chouaia, B., & Montagna, M. (2017). New insights into the microbiota of moth pests. International Journal of Molecular Sciences, 18(11), 2450. https://doi.org/10.3390/IJMS18112450
dc.relation.referencesMiles, M. A., Feliciangeli, M. D., & de Arias, A. R. (2003). American trypanosomiasis (Chagas disease) and the role of molecular epidemiology in guiding control strategies. BMJ (Clinical Research Ed.), 326(7404), 1444–1448. https://doi.org/10.1136/bmj.326.7404.1444
dc.relation.referencesMojahed, N., Mohammadkhani, M. A., & Mohamadkhani, A. (2022). Climate crises and developing vector-borne diseases: A narrative review. Iranian Journal of Public Health, 51(12), 2664–2673. https://doi.org/10.18502/ijph.v51i12.11457
dc.relation.referencesMontoya-Porras, L., Omar, T., Alzate, J., Moreno-Herrera, C., & Cadavid-Restrepo, G. (2018). 16S rRNA gene amplicon sequencing reveals dominance of Actinobacteria in Rhodnius pallescens compared to Triatoma maculata midgut microbiota in natural populations of vector insects from Colombia. Acta Tropica, 178, 327–332. https://doi.org/10.1016/j.actatropica.2017.11.004
dc.relation.referencesMoreno del Castillo, M. C., Valladares-García, J., & Halabe-Cherem, J. (2018). Microbioma humano. Revista de la Facultad de Medicina (México), 61(6), 7-19. https://doi.org/10.22201/fm.24484865e.2018.61.6.02
dc.relation.referencesMoser, D. R., Kirchhoff, L. V., & Donelson, J. E. (1989). Detection of Trypanosoma cruzi by DNA amplification using the polymerase chain reaction. Journal of Clinical Microbiology, 27(7), 1477-1482.
dc.relation.referencesMoser, D. R., Kirchhoff, L. V., & Donelson, J. E. (1989). Detection of Trypanosoma cruzi by DNA amplification using the polymerase chain reaction. Journal of Clinical Microbiology, 27(7), 1477-1482.
dc.relation.referencesMosqueda, E. (2023). What can modern bacteria and endosymbionts teach us about eukaryote mitochondria? Brazilian Journal of Animal and Environmental Research, 6(1), 469-471. https://doi.org/10.34188/bjaerv6n1-042
dc.relation.referencesMurillo-Solano, C., López-Domínguez, J., Gongora, R., et al. (2021). Diversity and interactions among triatomine bugs, their blood-feeding sources, gut microbiota and Trypanosoma cruzi in the Sierra Nevada de Santa Marta in Colombia. Scientific Reports, 11, 12306. https://doi.org/10.1038/s41598-021-91783-2
dc.relation.referencesNair, S. (2020). Dynamics of insect–microbiome interaction influence host and microbial symbiont. Front. Microbiol. 11:545024. doi: 10.3389/fmicb.2020.01357
dc.relation.referencesNational Center for Biotechnology Information (NCBI). (2024). BLASTn (Basic Local Alignment Search Tool). Disponible en: https://blast.ncbi.nlm.nih.gov/Blast.cgi
dc.relation.referencesNevoa, J.; Latorre-Estivalis, J. Sviatopolk-Mirsky, F. Pinto, P. da Rocha, M. Lorenzo, M. Guarneri, A. (2023). Global characterization of gene expression in the brain of starved immature Rhodnius prolixus https://doi.org/10.1371/journal.pone.0282490.
dc.relation.referencesOaks, S. C. Jr. (1991). Malaria: Obstacles and opportunities. National Institutes of Health (NIH). Recuperado dehttps://www.ncbi.nlm.nih.gov/books/NBK234235/
dc.relation.referencesOcaña-Mayorga, S., Bustillos, J. J., Villacís, A. G., Pinto, C. M., Brenière, S. F., & Grijalva, M. J. (2021). Triatomine Feeding Profiles and Trypanosoma cruzi Infection, Implications in Domestic and Sylvatic Transmission Cycles in Ecuador. Pathogens (Basel, Switzerland), 10(1), 42. https://doi.org/10.3390/pathogens10010042
dc.relation.referencesOliveira JL, Cury JC, Gurgel-Gonc ̧alves R, Bahia AC, Monteiro FA (2018) PLoS Negl Trop Dis 12(8): e0006709. https://doi.org/10.1371/journal.pntd.0006709
dc.relation.referencesOmondi, Z. N., Caner, A., & Arserim, S. K. (2024). Trypanosomes and gut microbiota interactions in triatomine bugs and tsetse flies: A vectorial perspective. Medical and veterinary entomology, 38(3), 253–268. https://doi.org/10.1111/mve.12723
dc.relation.referencesOMS. (2020). Enfermedades transmitidas por vectores. https://www.who.int/es/news-room/fact-sheets/detail/vector-borne-diseases
dc.relation.referencesOMS. (2025). Enfermedades transmitidas por vectores. https://www.who.int/es/news-room/fact-sheets/detail/vector-borne-diseases
dc.relation.referencesOnchuru, T. O., Makhulu, E. E., Ronnie, P. C., Mandere, S., Otieno, F. G., Gichuhi, J., & Herren, J. K. (2024). The Plasmodium transmission-blocking symbiont, Microsporidia MB, is vertically transmitted through Anopheles arabiensis germline stem cells. PLoS Pathogens, 20(11), e1012340. https://doi.org/10.1371/journal.ppat.1012340
dc.relation.referencesOPS (2017). Organización Panamericana de la Salud. arco para la eliminación de la transmisión maternoinfantil del VIH, sífilis, hepatitis B y Chagas. OPS/CHA/17- 009. Organización Panamericana de la Salud, Washington, DC. https://iris.paho.org/handle/10665.2/34306 . Consultado el 20 de diciembre de 2024.
dc.relation.referencesPadilla N, A., Moncayo, A. L., Keil, C. B., Grijalva, M. J., & Villacís, A. G. (2019). Life cycle, feeding, and defecation patterns of Triatoma carrioni (Hemiptera: Reduviidae), under laboratory conditions. Journal of Medical Entomology, 56(3), 617–624. https://doi.org/10.1093/jme/tjz004
dc.relation.referencesParra-Henao, G., Garzón-Jiménez, S. P., Bernal-Rosas, Y., Olivera, M. J., Salgado, M., & Torres-García, O. A. (2021). Risk factors for Triatominae infestation in a municipality of Colombia. Therapeutic Advances in Infectious Disease, 8, 20499361211030068. https://doi.org/10.1177/20499361211030068
dc.relation.referencesParra-Henao, G., Suárez-Escudero, L. C., & González-Caro, S. (2016). Potential distribution of Chagas disease vectors (Hemiptera, Reduviidae, Triatominae) in Colombia, based on ecological niche modeling. Journal of Tropical Medicine, 2016, 1439090. https://doi.org/10.1155/2016/1439090
dc.relation.referencesPatiño, L. H., Castillo-Castañeda, A. C., Muñoz, M., Jaimes, J. E., Luna-Niño, N., Hernández, C., Ayala, M. S., Fuya, P., Mendez, C., Hernández-Pereira, C. E., Delgado, L., Sandoval-Ramírez, C. M., Urbano, P., Paniz-Mondolfi, A., & Ramírez, J. D. (2021). Development of an amplicon-based next-generation sequencing protocol to identify Leishmania species and other trypanosomatids in leishmaniasis endemic areas. Microbiology Spectrum, 9(2), e0065221. https://doi.org/10.1128/Spectrum.00652-21
dc.relation.referencesPavía, P. X., Cuervo, C. L., Gil, J., Romero, I., Morales, L., Díez, H., Quintero, C., del Portillo, P., Vallejo, G. A., Florez, A. C., Montilla, M., Mercado, M., Vacca, M., Nicholls, R. S., López, M. C., & Puerta, C. J. (2009). Caracterización molecular de los genes histona H2A y ARNsno-Cl de Trypanosoma rangeli: Aplicación en pruebas diagnósticas. Infectio, 13(1), 43-52.
dc.relation.referencesPeña, G., González, J., Jiménez, J., Fuentes, J., Salazar, P., Bucio, M., Cabrera, M., & Flores, A. (2022). Enfermedad de Chagas: Biología y transmisión de Trypanosoma cruzi. TIP Revista Especializada en Ciencias Químico-Biológicas, 25(0), 1–19. https://doi.org/10.22201/FESZ.23958723E.2022.449
dc.relation.referencesQi, Y., Zhang, J., André, M. R., & Qin, T. (2024). Editorial: New insights in the microbe-vector interaction. Frontiers in Microbiology, 15, 1364989. https://doi.org/10.3389/fmicb.2024.1364989
dc.relation.referencesRafiqi, A. M., Polo, P. G., Milat, N. S., Durmuş, Z. Ö., Çolak-Al, B., Alarcón, M. E., Çağıl, F. Z., & Rajakumar, A. (2022). Developmental integration of endosymbionts in insects. Frontiers in Ecology and Evolution, 10, 846586. https://doi.org/10.3389/fevo.2022.846586
dc.relation.referencesRassi, A., Rassi, A., & Marin, J. (2010). Chagas disease. The Lancet, 375(9723), 1388–1402. https://doi.org/10.1016/S0140-6736(10)60061-X
dc.relation.referencesReyes, L., Romero, C., & Heredia, R. (2020). Evaluación de enfermedades transmitidas por vectores en perros de un área de clima sub-frío de México. Acta Biológica Colombiana, 25(2), 219–224. https://doi.org/10.15446/abc.v25n2.77737
dc.relation.referencesRoa, A., Gaitán, X., Eresbey, Y., Clavijo, J., Teixeira, M., Montilla, M., Vallejo, G., & Carranza, J. (2013). Capacidad vectorial de Rhodnius colombiensis para transmitir Trypanosoma cruzi I y T. cruzi II. Revista de la Asociación Colombiana de Ciencias Biológicas, 1(25), 22–30. https://www.revistaaccb.org/r/index.php/accb/article/view/
dc.relation.referencesRodrigues, J. M. D. S., da Rosa, J. A., Moreira, F. F. F., & Galvão, C. (2018). Morphology of the terminal abdominal segments in females of Triatominae (Insecta: Hemiptera: Reduviidae). Acta Tropica, 185, 86–97. https://doi.org/10.1016/j.actatropica.2018.04.021
dc.relation.referencesRodríguez, E., Cantillo, O., Prieto, A., & Cucunubá, M. (2019). Heterogeneidad de Trypanosoma cruzi, tasas de infección en vectores y reservorios animales en Colombia: una revisión sistemática y metanálisis. Parasites & Vectors, 12, 308. https://doi.org/10.1186/s13071-019-3541-5
dc.relation.referencesRodríguez, S., Škochová, V., Rego, R., Schmidt, J., Roachell, W., Hypša, V., & Nováková, E. (2018). Microbiomes of North American Triatominae: The grounds for Chagas disease epidemiology. Frontiers in Microbiology, 9, 1167. https://doi.org/10.3389/fmicb.2018.01167
dc.relation.referencesRodríguez-Ruano, S. M., Škochová, V., Rego, R. O. M., Schmidt, J. O., Roachell, W., Hypša, V., & Nováková, E. (2018). Microbiomes of North American Triatominae: The Grounds for Chagas Disease Epidemiology. Frontiers in microbiology, 9, 1167. https://doi.org/10.3389/fmicb.2018.01167
dc.relation.referencesRojas, J. D., Pereira, M., Martínez, B., Gómez, J. C., & Cuervo, S. I. (2022). Chagas disease reactivation after autologous stem cell transplant: Case report and literature review. Biomédica, 42(2), 224–233. https://doi.org/10.7705/biomedica.6288
dc.relation.referencesRos, V. I. D., Fleming, V. M., Feil, E. J., & Breeuwer, J. A. J. (2012). Diversity and recombination in Wolbachia and Cardinium from Bryobia spider mites. BMC Microbiology, 12(S13). https://doi.org/10.1186/1471-2180-12-S1-S13
dc.relation.referencesRozas-Dennis, G. S., La Torre, J. L., Muscio, O. A., & Guérin, D. M. A. (2000). Direct methods for detecting picorna-like virus from dead and alive triatomine insects. Memórias do Instituto Oswaldo Cruz, 95(3), 323–327. https://www.scielo.br/j/mioc/a/kxk3sHCfnTpbHTczyCrcRcB/?format=pdf
dc.relation.referencesRueda, K., Trujillo, J. E., Carranza, J. C., & Vallejo, G. A. (2014). Transmisión oral de Trypanosoma cruzi: Una nueva situación epidemiológica de la enfermedad de Chagas en Colombia y otros países suramericanos. Biomédica, 34, 631–672. https://doi.org/10.7705/biomedica.v34i4.2204
dc.relation.referencesSalcedo-Porras, N., Umaña-Diaz, C., Bitencourt, R. O. B., & Lowenberger, C. (2020). The role of bacterial symbionts in triatomines: An evolutionary perspective. Microorganisms, 8(9), 1438. https://doi.org/10.3390/microorganisms8091438
dc.relation.referencesSant’Anna, M. R. V., Soares, A. C., Araujo, R. N., Gontijo, N. F., & Pereira, M. H. (2016). Triatomines (Hemiptera, Reduviidae) blood intake: Physical constraints and biological adaptations. Journal of Insect Physiology, 91-92, 20–26. https://doi.org/10.1016/j.jinsphys.2016.08.004
dc.relation.referencesSantos, D., Gontijo, N., Pessoa, G., Sant’Anna, M., Araujo, R., Pereira, M., & Koerich, L. (2022). An updated catalog of lipocalins of the Chagas disease vector Rhodnius prolixus (Hemiptera, Reduviidae). Insect Biochemistry and Molecular Biology, 146, 103797. https://doi.org/10.1016/j.ibmb.2022.103797
dc.relation.referencesSavić, S., Vidić, B., Grgić, Z., Potkonjak, A., & Spasojević, L. (2014). Emerging vector-borne diseases - Incidence through vectors. Frontiers in Public Health, 2, 267. https://doi.org/10.3389/fpubh.2014.00267
dc.relation.referencesSchaub, G. A. (1989). Trypanosoma cruzi: Quantitative studies of development of two strains in small intestine and rectum of the vector Triatoma infestans. Experimental Parasitology, 68(3), 260–273. https://doi.org/10.1016/0014-4894(89)90108-2
dc.relation.referencesSchaub, G. A. (2021). An update on the knowledge of parasite-vector interactions of Chagas disease. Research Reports in Tropical Medicine, 12, 63–76.
dc.relation.referencesSchaub, G. A. (2024). Interaction of Trypanosoma cruzi, triatomines and the microbiota of the vectors - A review. Microorganisms, 12(5), 855. https://doi.org/10.3390/microorganisms12050855
dc.relation.referencesSchaub, G. A., & Jensen, C. (1990). Developmental time and mortality of the reduviid bug Triatoma infestans with differential exposure to coprophagic infections with Blastocrithidia triatomae (Trypanosomatidae). Journal of Invertebrate Pathology, 55(2), 231–238. https://doi.org/10.1016/0022-2011(90)90027-4
dc.relation.referencesShaw, J. J., Marinho-Júnior, J. F., Courtenay, O., & Brandão-Filho, S. P. (2023). Assessing reservoir host status in leishmaniasis with special reference to the infectiousness of Leishmania (Viannia) braziliensis infections in wild rodents. Revista da Sociedade Brasileira de Medicina Tropical, 56, 0503. https://doi.org/10.1590/0037-8682-0503-2023
dc.relation.referencesSilvestrini, M. M. A., Alessio, G. D., Frias, B. E. D., Sales Júnior, P. A., Araújo, M. S. S., Silvestrini, C. M. A., Brito Alvim de Melo, G. E., Martins-Filho, O. A., Teixeira-Carvalho, A., & Martins, H. R. (2024). New insights into Trypanosoma cruzi genetic diversity, and its influence on parasite biology and clinical outcomes. Frontiers in Immunology, 15, 1342431. https://doi.org/10.3389/fimmu.2024.1342431
dc.relation.referencesSoto-Vivas, A., Liria, J., & De Luna, E. (2011). Morfometría geométrica y filogenia en Rhodniini (Hemiptera, Reduviidae) de Venezuela. Acta Zoológica Mexicana, 27(1), 87-102.
dc.relation.referencesSparks, J., Bohbot, J., & Dickens, J. (2015). Olfactory disruption: Toward controlling important insect vectors of disease. Progress in Molecular Biology and Translational Science, 130, 81–108. https://doi.org/10.1016/bs.pmbts.2014.11.004
dc.relation.referencesSteverding, D., Sidjui, L. S., Ferreira, É. R., Ngameni, B., Folefoc, G. N., Mahiou-Leddet, V., Ollivier, E., Stephenson, G. R., Storr, T. E., & Tyler, K. M. (2020). Trypanocidal and leishmanicidal activity of six limonoids. Journal of Natural Medicines, 74, 606–611. https://doi.org/10.1007/s11418-020-01408-7
dc.relation.referencesSuárez-Quevedo, Y., Barbosa-Vinasco, H. J., Gutiérrez-Garnizo, S. A., Olaya-Morales, J. L., Zabala-González, D., Carranza-Martínez, J. C., Guhl-Nannetti, F., Cantillo-Barraza, O., & Vallejo, G. A. (2020). Innate trypanolytic factors in triatomine hemolymph against Trypanosoma rangeli and T. cruzi: A comparative study in eight Chagas disease vectors. Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales, 44(170), 88-104. https://doi.org/10.18257/raccefyn.1097
dc.relation.referencesTagliavia, M., Messina, E., Manachini, B., & et al. (2014). The gut microbiota of larvae of Rhynchophorus ferrugineus Oliver (Coleoptera: Curculionidae). BMC Microbiology, 14, 136. https://doi.org/10.1186/1471-2180-14-136
dc.relation.referencesTamura, K., Stecher, G., & Kumar, S. (2021). MEGA11: Molecular Evolutionary Genetics Analysis version 11. Molecular Biology and Evolution, 38(7), 3022–3027. https://doi.org/10.1093/molbev/msab120
dc.relation.referencesTarabai, H., Floriano, A. M., Zima, J., Filová, N., Brown, J. J., Roachell, W., Smith, R. L., Beatty, N. L., Vogel, K. J., & Nováková, E. (2023). Microbiomes of blood-feeding triatomines in the context of their predatory relatives and the environment. Microbiology Spectrum, 11, e01681-23. https://doi.org/10.1128/spectrum.01681-23
dc.relation.referencesTarlachkov, S. V., Efeykin, B. D., Castillo, P., Evtushenko, L. I., & Subbotin, S. A. (2023). Distribution of bacterial endosymbionts of the Cardinium clade in plant-parasitic nematodes. International Journal of Molecular Sciences, 24(3), 2905. https://doi.org/10.3390/ijms24032905
dc.relation.referencesTeal, E., Herrera, C., & Dumonteil, E. (2023). Metabolomics of developmental changes in Triatoma sanguisuga gut microbiota. PLOS ONE, 18(2), e0280868. https://doi.org/10.1371/journal.pone.0280868
dc.relation.referencesTeixeira, D., Benchimol, M., Crepaldi, P., & de Souza, W. (2012). Interactive multimedia to teach the life cycle of Trypanosoma cruzi, the causative agent of Chagas disease. PLOS Neglected Tropical Diseases, 6(8), e1749. https://doi.org/10.1371/journal.pntd.0001749
dc.relation.referencesTercero, M. J., & Olalla, R. (2008). Hidatidosis: Una zoonosis de distribución mundial. Offarm, 27(9), 88–94. https://www.elsevier.es/es-revista-offarm-4-articulohidatidosis-una-zoonosis-distribucion-mundial-13127387
dc.relation.referencesTinker, K. A., & Ottesen, E. A. (2021). Differences in gut microbiome composition between sympatric wild and allopatric laboratory populations of omnivorous cockroaches. Frontiers in Microbiology, 12, 703785. https://doi.org/10.3389/fmicb.2021.703785
dc.relation.referencesUribe-Álvarez, C., & Chiquete, N. (2017). Las enfermedades transmitidas por vectores y el potencial uso de Wolbachia, una bacteria endocelular obligada, para erradicarlas. Revista de la Facultad de Medicina (México), 60(6), 51–55. Recuperado el 20 de noviembre de 2024, de http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0026-17422017000600051&lng=es&tlng=es
dc.relation.referencesVallejo, G. A., Marinkelle, C. J., Guhl, F., & Sánchez, N. (1988). Comportamiento de la infección y diferenciación morfológica entre Trypanosoma cruzi y T. rangeli en el intestino del vector Rhodnius prolixus. Revista Brasileira de Biologia, 48(3), 577-587.
dc.relation.referencesVallejo, G. A., Suárez, Y., Olaya, J. L., Gutiérrez, S. A., & Carranza, J. C. (2015). Trypanosoma rangeli: Un protozoo infectivo y no patógeno para el humano que contribuye al entendimiento de la transmisión vectorial y la infección por Trypanosoma cruzi, agente causal de la enfermedad de Chagas. Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales, 39(150), 111-122.
dc.relation.referencesVelásquez-Ortiz, N., Herrera, G., Hernández, C., Muñoz, M., & Ramírez, J. D. (2022). Discrete typing units of Trypanosoma cruzi: Geographical and biological distribution in the Americas. Scientific Data, 9, 360. https://doi.org/10.1038/s41597-022-01452-w
dc.relation.referencesVieira, C. B., Praça, Y. R., Bentes, K. L. D. S., Santiago, P. B., Silva, S. M. M., Silva, G. D. S., Motta, F. N., Bastos, I. M. D., de Santana, J. M., & de Araújo, C. N. (2018). Triatomines: Trypanosomatids, bacteria, and viruses potential vectors? Frontiers in Cellular and Infection Microbiology, 8, 405. https://doi.org/10.3389/fcimb.2018.00405
dc.relation.referencesVillacís, J. F., López-Rosero, A., Bustillos, J. J., Cadena, M., Yumiseva, C. A., Grijalva, M. J., & Villacís, A. G. (2024). Bacterial microbiota from the gut of Rhodnius ecuadoriensis, a vector of Chagas disease in Ecuador's Central Coast and Southern Andes. Frontiers in Microbiology, 15. https://doi.org/10.3389/fmicb.2024.1464720
dc.relation.referencesVivas, R. (2020). Efecto de la temperatura sobre el ciclo de vida, la reproducción y la capacidad vectorial (metaciclogénesis) de Panstrongylus geniculatus [Trabajo de grado, Universidad del Tolima].
dc.relation.referencesVivero, R. J., Cadavid-Restrepo, G., Herrera, C., & Soto, S. (2017). Molecular detection and identification of Wolbachia in three species of the genus Lutzomyia on the Colombian Caribbean coast. Parasites & Vectors, 10(1), 110. https://doi.org/10.1186/S13071-017-2031-X
dc.relation.referencesWalter, A., Lozano-Kasten, F., Bosseno, M. F., Ruvalcaba, E. G., Gutierrez, M. S., Luna, C. E., Baunaure, F., Phélinas, P., Magallón-Gastélum, E., & Brenière, S. F. (2007). Peridomicilary habitat and risk factors for Triatoma infestation in a rural community of the Mexican occident. The American journal of tropical medicine and hygiene, 76(3), 508–515.
dc.relation.referencesWaltmann, A., Willcox, A. C., Balasubramanian, S., Borrini Mayori, K., Mendoza Guerrero, S., Salazar Sanchez, R. S., Roach, J., Condori Pino, C., Gilman, R. H., Bern, C., Juliano, J. J., Levy, M. Z., Meshnick, S. R., & Bowman, N. M. (2019). Hindgut microbiota in laboratory-reared and wild Triatoma infestans. PLOS Neglected Tropical Diseases, 13(5), e0007383. https://doi.org/10.1371/journal.pntd.0007383
dc.relation.referencesWang, J., Gao, L., & Aksoy, S. (2023). Microbiota in disease-transmitting vectors. Nature reviews. Microbiology, 21(9), 604–618. https://doi.org/10.1038/s41579-023-00901-6
dc.relation.referencesWilke, A., Benelli, G., & Beier, J. (2021). Anthropogenic changes and associated impacts on vector-borne diseases. Trends in Parasitology, 37(12), 1027–1030. https://doi.org/10.1016/j.pt.2021.09.013
dc.relation.referencesWeiss, B., & Aksoy, S. (2011). Microbiome influences on insect host vector competence. Trends in Parasitology, 27(11), 514–522. https://doi.org/10.1016/J.PT.2011.05.001
dc.relation.referencesWigglesworth, V. B. (1936). Bacteria simbiótica en un insecto chupador de sangre, Rhodnius prolixus Stal. (Hemípteros, Triatomidae). Parasitología, 28, 284–289. https://doi.org/10.1017/S0031182000022459
dc.relation.referencesWorld Health Organization. (2024, April 4). Chagas disease (American trypanosomiasis). https://www.who.int/news-room/fact-sheets/detail/chagas-disease-(american-trypanosomiasis)
dc.relation.referencesXiong, Q., Fung, C. S., Xiao, X., Wan, A. T., Wang, M., Klimov, P., Ren, Y., Yang, K. Y., Hubert, J., Cui, Y., Liu, X., & Tsui, S. K. (2023). Endogenous plasmids and chromosomal genome reduction in the Cardinium endosymbiont of Dermatophagoides farinae. mSphere, 8(e00074-23). https://doi.org/10.1128/msphere.00074-23
dc.relation.referencesYeates, D. K., Seago, A., Nelson, L., & Cameron, S. L. (2010). Integrative taxonomy, or iterative taxonomy? Systematic Entomology, 36(2), 209–217. https://doi.org/10.1111/j.1365-3113.2010.00558.x
dc.relation.referencesZeng, T., Su, H. A., Liu, Y. L., Li, J. F., Jiang, D. X., Lu, Y. Y., & Qi, Y. X. (2022a). Serotonin modulates insect gut bacterial community homeostasis. BMC Biology, 20(1), 105. https://doi.org/10.1186/s12915-022-01319-x
dc.relation.referencesZeng, X., Xing, X., Gupta, M., Keber, F. C., Lopez, J. G., Lee, Y. J., Roichman, A., Wang, L., Neinast, M. D., Donia, M. S., Wühr, M., Jang, C., & Rabinowitz, J. D. (2022b). Gut bacterial nutrient preferences quantified in vivo. Cell, 185(18), 3441–3456.e19. https://doi.org/10.1016/j.cell.2022.07.020
dc.relation.referencesZhou, W., Rousset, F., & O’Neill, S. (1998). Phylogeny and PCR-based classification of Wolbachia strains using wsp gene sequences. Proceedings. Biological Sciences, 265(1395), 509–515. https://doi.org/10.1098/RSPB.1998.0324
dc.relation.referencesZhu, Y., & Wang, J. (2024). Editorial overview: Vectors, symbionts, and pathogen interactions: Prospects for controlling vector-borne diseases. Current Opinion in Insect Science, 64, 101232. https://doi.org/10.1016/j.cois.2024.101232
dc.relation.referencesZingales, B., Andrade, S. G., Briones, M. R., Campbell, D. A., Chiari, E., Fernandes, O., Guhl, F., Lages-Silva, E., Macedo, A. M., Machado, C. R., Miles, M. A., Romanha, A. J., Sturm, N. R., Tibayrenc, M., Schijman, A. G., & Second Satellite Meeting. (2009). A new consensus for Trypanosoma cruzi intraspecific nomenclature: Second revision meeting recommends TcI to TcVI. Memórias do Instituto Oswaldo Cruz, 104(7), 1051–1054.
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.rights.licenseAtribución-NoComercial-CompartirIgual 4.0 Internacional
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.subject.ddc660 - Ingeniería química
dc.subject.lembEnfermedad de Chagas
dc.subject.lembTrypanosoma cruzi
dc.subject.lembControl de insectos
dc.subject.proposalTripanosomiasis Americanaspa
dc.subject.proposalTriatominaespa
dc.subject.proposalMicrobiotaspa
dc.subject.proposalEndosimbiontesspa
dc.subject.proposalReservoriosspa
dc.subject.proposalAmerican trypanosomiasiseng
dc.subject.proposalTriatominaeeng
dc.subject.proposalMicrobiotaeng
dc.subject.proposalEndosymbiontseng
dc.subject.proposalReservoirseng
dc.subject.wikidataMicrobiota
dc.titleEstudio del microbioma asociado a insectos vectores triatominos en zonas de transmisión de la enfermedad de Chagasspa
dc.title.translatedStudy of the microbiome associated with triatomine insect vectors in Chagas disease transmission areaseng
dc.typeTrabajo de grado - Maestría
dc.type.coarhttp://purl.org/coar/resource_type/c_bdcc
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.contentText
dc.type.driverinfo:eu-repo/semantics/masterThesis
dc.type.redcolhttp://purl.org/redcol/resource_type/TM
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dcterms.audience.professionaldevelopmentEstudiantes
dcterms.audience.professionaldevelopmentInvestigadores
oaire.accessrightshttp://purl.org/coar/access_right/c_abf2

Archivos

Bloque original

Mostrando 1 - 1 de 1
Miniatura
Nombre:
Tesis de Maestría en Ciencias - Biotecnología.pdf
Tamaño:
3.65 MB
Formato:
Adobe Portable Document Format
Descargar

Bloque de licencias

Mostrando 1 - 1 de 1
Miniatura
Nombre:
license.txt
Tamaño:
5.74 KB
Formato:
Item-specific license agreed upon to submission
Descripción:
Descargar

Colecciones

Maestría en Ciencias - Biotecnología