Patrones de expresión de Notch1, Serrate2 y genes diana de la vía Notch en prominencias faciales de embriones de pollo (Gallus gallus)
Autores
Carbonell Medina, Belfran Alcides
Director
Parada Borja, Carolina (Thesis advisor)
Garavito Aguilar, Zayra (Thesis advisor)
Tipo de contenido
Trabajo de grado - Maestría
Idioma del documento
EspañolFecha de publicación
2012
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Resumen
La vía de señalización Notch desempeña un papel fundamental en diferentes etapas del desarrollo embrionario y está involucrada en diversos procesos celulares como apoptosis, proliferación, diferenciación, decisión de destino celular y mantenimiento de células indiferenciadas. Mutaciones en varios genes componentes de la vía Notch, como Notch1, Jagged2 (Serrate2) y Hes1 producen alteraciones en el desarrollo de estructuras craneofaciales como paladar, bóveda craneal y maxilares. El objetivo del presente estudio fue determinar los patrones de expresión de los genes Notch1, Serrate2, Hes1 y Hey1 durante el desarrollo de prominencias maxilares, mandibulares y frontonasal durante los estadios de desarrollo HH19, HH21, HH23 y HH25 en un modelo animal de pollo. Los resultados muestran expresión de Notch1, Serrate2 y Hey1, en el epitelio nasal al igual que en el mesénquima y epitelio que recubre las prominencias maxilar y mandibular. Adicionalmente, Notch1 y Hey1 también se expresan en el ectodermo frontonasal en HH21 y en condensaciones mesénquimales en la prominencia mandibular en HH25. La expresión de Hes1 está restringida al epitelio maxilar y mandibular en HH19 y HH21. En conjunto los resultados sugieren un potencial papel de la vía Notch durante el desarrollo de las prominencias faciales. Por lo tanto, se sugieren futuros estudios de pérdida y ganancia de función para identificar el rol específico de esta vía de señalización durante la morfogénesis facial.
Abstract. The Notch signaling pathway plays an essential role during embryonic development and has been implicated in diverse cellular processes such as apoptosis, proliferation, differentiation, and cell fate decision maintenance of undifferentiated cells. Mutations in several Notch pathway component genes, including Notch1, Jagged2 (Serrate2) and Hes1 lead to alterations in craniofacial structures, palate, maxillary and cranial vault. The aim of this study was to analyze the Notch1, Serrate2, Hes1 and Hey1 gene expression patterns during development of maxillary, mandibular and frontonasal prominences during developmental stages HH19, HH21, HH23 and HH25 in chick embryo animal model. The results show notch1, serrate2 and hey1 expression in the nasal epithelium as well as in the ectoderm-derived epithelium and lateral mesenchyme of maxillary and mandibular prominences. Additionally, Notch1 and Hey1 are also expressed in the frontonasal ectoderm at HH21 and the mesenchymal condensations of the mandibular prominence at HH25. Hes1 expression was restricted to the maxillary and mandibular ectoderm at HH19 and HH21. Overall the results suggest a potential role of the Notch pathway in the development of facial prominences. Therefore, future studies of loss and gain of function are suggested to identify the specific roles of this signaling pathway during facial morphogenesis.
Abstract. The Notch signaling pathway plays an essential role during embryonic development and has been implicated in diverse cellular processes such as apoptosis, proliferation, differentiation, and cell fate decision maintenance of undifferentiated cells. Mutations in several Notch pathway component genes, including Notch1, Jagged2 (Serrate2) and Hes1 lead to alterations in craniofacial structures, palate, maxillary and cranial vault. The aim of this study was to analyze the Notch1, Serrate2, Hes1 and Hey1 gene expression patterns during development of maxillary, mandibular and frontonasal prominences during developmental stages HH19, HH21, HH23 and HH25 in chick embryo animal model. The results show notch1, serrate2 and hey1 expression in the nasal epithelium as well as in the ectoderm-derived epithelium and lateral mesenchyme of maxillary and mandibular prominences. Additionally, Notch1 and Hey1 are also expressed in the frontonasal ectoderm at HH21 and the mesenchymal condensations of the mandibular prominence at HH25. Hes1 expression was restricted to the maxillary and mandibular ectoderm at HH19 and HH21. Overall the results suggest a potential role of the Notch pathway in the development of facial prominences. Therefore, future studies of loss and gain of function are suggested to identify the specific roles of this signaling pathway during facial morphogenesis.